A maturation-related differential phosphorylation of the plasma membrane proteins of the epididymal spermatozoa of the hamster by endogenous protein kinases

Molecular Reproduction and Development
K U DeviSisinthy Shivaji

Abstract

When the plasma membranes of caput and cauda epididymal spermatozoa of hamster were evaluated for their ability to undergo phosphorylation, a differential phosphorylation of the membrane proteins was observed. In the plasma membranes of the caput epididymal spermatozoa (immature), twelve proteins were phosphorylated (100, 76, 67, 65, 55, 52, 47, 42, 38, 32, 30, and 20 kD), whereas in the plasma membranes of cauda epididymal spermatozoa (mature), a differential phosphorylation pattern was observed with respect to the 94, 67, 52, and 47 kD proteins. The 94 kD protein was found to be phosphorylated and the 67 kD protein was found to be not phosphorylated in cauda spermatozoal plasma membrane (Cd SPM) in contrast to this protein in caput spermatozoal plasma membrane (Cpt SPM). The 52 and 47 kD proteins were also more intensely phosphorylated in Cd SPM than Cpt SPM. The 100 kilodalton protein, although present in both Cpt and Cd sperm plasma membranes, was found to be phosphorylated at the tyrosine residues only in the Cd SPM, as indicated by the Western blot using antiphosphotyrosine antibody. Further, a differential phosphorylation of the substrate proteins present in the Cpt and Cd SPM was seen when Mg2+ in the assay buffer was r...Continue Reading

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Citations

May 21, 2004·Biology of Reproduction·Judith SeligmanNechama S Kosower
Dec 5, 2012·Journal of Signal Transduction·Takashi W IjiriKen-Ichi Sato
Jan 5, 2005·Toxicology and Applied Pharmacology·B Piña-GuzmánB Quintanilla-Vega
Feb 20, 1999·Biology of Reproduction·L R JohnsonG L Gerton
Sep 22, 2001·Biology of Reproduction·P VernetR J Aitken
May 7, 1999·Molecular Reproduction and Development·K U DeviS Shivaji
Apr 28, 2004·Molecular Reproduction and Development·Archana B SivaSisinthy Shivaji
Nov 7, 2000·Biochimica Et Biophysica Acta·F M Flesch, B M Gadella

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