A mechanism distinct from highwire for the Drosophila ubiquitin conjugase bendless in synaptic growth and maturation.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Smitha B UthamanRodney K Murphey

Abstract

The signaling mechanisms that allow the conversion of a growth cone into a mature and stable synapse are yet to be completely understood. Ubiquitination plays key regulatory roles in synaptic development and may be involved in this process. Previous studies identified the Drosophila ubiquitin conjugase bendless (ben) to be important for central synapse formation, but the precise role it plays has not been elucidated. Our studies indicate that Ben plays a pivotal role in synaptic growth and maturation. We have determined that an incipient synapse is present with a high penetrance in ben mutants, suggesting that Ben is required for a developmental step after target recognition. We used cell-autonomous rescue experiments to show that Ben has a presynaptic role in synapse growth. We then harnessed the TARGET system to transiently express UAS (upstream activating sequence)-ben in a ben mutant background and identified a well defined critical period for Ben function in establishing a full-grown, mature synaptic terminal. We demonstrate that the protein must be present at a time point before but not during the actual growth process. We also provide phenotypic evidence demonstrating that Ben is not a part of the signal transduction pat...Continue Reading

References

Apr 1, 1993·Journal of Neurogenetics·R S EdgecombA M Schneiderman
Aug 12, 1998·The Journal of Comparative Neurology·M J AllenK G Moffat
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jun 6, 2000·Neuron·H I WanC S Goodman
Sep 19, 2001·The Journal of Biological Chemistry·C BüttnerG Schmalzing
Oct 9, 2002·Neuron·Rodney K Murphey, Tanja A Godenschwege
Dec 5, 2003·Current Biology : CB·Gentry N PatrickErin M Schuman
Dec 6, 2003·Science·Sean E McGuireRonald L Davis
Feb 19, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sean E McGuireRonald L Davis
Mar 30, 2004·Neuron·Brian D McCabeA Pejmun Haghighi
Apr 22, 2004·Nature Reviews. Neuroscience·Noam E Ziv, Craig C Garner
Jun 26, 2004·Annual Review of Neuroscience·Aaron DiAntonio, Linda Hicke
Jul 21, 2004·Trends in Genetics : TIG·Sean E McGuireRonald L Davis
Apr 19, 2005·Genetics·Matthew J LavioletteBryan A Stewart
Oct 21, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chunlai WuAaron DiAntonio
Jan 13, 2006·Current Biology : CB·Tanja A GodenschwegeRodney K Murphey
Feb 18, 2006·Science·Asim A Beg, Peter Scheiffele
Jan 24, 2007·Trends in Neurosciences·Astrid ScheschonkaHeinrich Betz
May 9, 2007·Nature·Françoise Coussen, Daniel Choquet
May 9, 2007·Nature·Stéphane MartinJeremy M Henley
Jul 24, 2007·Nature Neuroscience·Eunju Pack-ChungThomas L Schwarz
Oct 30, 2007·Developmental Neurobiology·Faith L W LieblHuey Hing
Dec 29, 2007·Developmental Neurobiology·Sara Seabrooke, Bryan A Stewart
Jan 29, 2008·Biochimica Et Biophysica Acta·Kevin F Haas, Kendal Broadie
Jan 29, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Benjamin AbramsYishi Jin
Feb 8, 2008·Neuron·Tudor A Fulga, David Van Vactor

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Citations

Oct 29, 2013·Cell Death and Differentiation·X MaT Xu
Aug 17, 2011·Cell Research·Xianjue MaLei Xue
Jul 22, 2010·Cold Spring Harbor Protocols·Marcus J Allen, Tanja A Godenschwege
Jun 16, 2011·Journal of Visualized Experiments : JoVE·Jana Boerner, Tanja A Godenschwege
Aug 28, 2004·Trends in Neurosciences·Brian D Ackley, Yishi Jin
Aug 4, 2016·Oncotarget·Curtis D HodgeJ N Mark Glover
Sep 24, 2010·Molecular & Cellular Proteomics : MCP·Maribel FrancoUgo Mayor

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