PMID: 9188574Jul 1, 1997Paper

A mechanism for negative gene regulation in Autographa californica multinucleocapsid nuclear polyhedrosis virus

Journal of Virology
D J LeisyG F Rohrmann

Abstract

The Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) ie-1 gene product (IE-1) is thought to play a central role in stimulating early viral transcription. IE-1 has been demonstrated to activate several early viral gene promoters and to negatively regulate the promoters of two other AcMNPV regulatory genes, ie-0 and ie-2. Our results indicate that IE-1 negatively regulates the expression of certain genes by binding directly, or as part of a complex, to promoter regions containing a specific IE-1-binding motif (5'-ACBYGTAA-3') near their mRNA start sites. The IE-1 binding motif was also found within the palindromic sequences of AcMNPV homologous repeat (hr) regions that have been shown to bind IE-1. The role of this IE-1 binding motif in the regulation of the ie-2 and pe-38 promoters was examined by introducing mutations in these promoters in which the central 6 bp were replaced with BglII sites. GUS reporter constructs containing ie-2 and pe-38 promoter fragments with and without these specific mutations were cotransfected into Sf9 cells with various amounts of an ie-1-containing plasmid (pIe-1). Comparisons of GUS expression produced by the mutant and wild-type constructs demonstrated that the IE-1 bi...Continue Reading

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Citations

Oct 21, 2010·Virologica Sinica·Xiao-wei MeiZhong-xin Zhang
Aug 9, 2006·The Journal of General Virology·Motoko IkedaMichihiro Kobayashi
Jul 12, 2007·The Journal of General Virology·Catherine Y Y LiuYu Chan Chao

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