A Mechanism of Unidirectional Transformation, Leading to Antibiotic Resistance, Occurs within Nasopharyngeal Pneumococcal Biofilm Consortia

MBio
Santiago M LattarJorge E Vidal

Abstract

Streptococcus pneumoniae acquires genes for resistance to antibiotics such as streptomycin (Str) or trimethoprim (Tmp) by recombination via transformation of DNA released by other pneumococci and closely related species. Using naturally transformable pneumococci, including strain D39 serotype 2 (S2) and TIGR4 (S4), we studied whether pneumococcal nasopharyngeal transformation was symmetrical, asymmetrical, or unidirectional. Incubation of S2Tet and S4Str in a bioreactor simulating the human nasopharynx led to the generation of SpnTet/Str recombinants. Double-resistant pneumococci emerged soon after 4 h postinoculation at a recombination frequency (rF) of 2.5 × 10-4 while peaking after 8 h at a rF of 1.1 × 10-3 Acquisition of antibiotic resistance genes by transformation was confirmed by treatment with DNase I. A high-throughput serotyping method demonstrated that all double-resistant pneumococci belonged to one serotype lineage (S2Tet/Str) and therefore that unidirectional transformation had occurred. Neither heterolysis nor availability of DNA for transformation was a factor for unidirectional transformation given that the density of each strain and extracellular DNA (eDNA) released from both strains were similar. Unidirection...Continue Reading

References

Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·L S HåvarsteinD A Morrison
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Karin L MeibomGary K Schoolnik
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sébastien GuiralJean-Pierre Claverys
Dec 17, 2005·Science·Karin L MeibomGary K Schoolnik
Mar 3, 2007·FEMS Microbiology Letters·Alexander HalfmannReinhold Brückner
Jun 1, 2007·Journal of Clinical Microbiology·Maria da Gloria S CarvalhoJacquelyn S Sampson
Mar 15, 2008·Nature Reviews. Microbiology·Aras KadiogluPeter W Andrew
Feb 21, 2009·FEMS Microbiology Reviews·Jean-Pierre ClaverysPatrice Polard
Sep 15, 2009·Lancet·Katherine L O'BrienUNKNOWN Hib and Pneumococcal Global Burden of Disease Study Team
Oct 8, 2009·American Journal of Epidemiology·Orin S Levine, Keith P Klugman
Nov 3, 2009·Lancet·Tom van der Poll, Steven M Opal
Jan 1, 1928·The Journal of Hygiene·F Griffith
Jan 29, 2011·Science·Nicholas J CroucherStephen D Bentley
Jun 19, 2012·Applied and Environmental Microbiology·Hua Wei, Leiv Sigve Håvarstein
Jun 22, 2012·PLoS Pathogens·Nicholas J CroucherStephen D Bentley
Jan 1, 2013·Trends in Microbiology·Joshua R ShakKeith P Klugman
Feb 11, 2014·Nature Genetics·Claire ChewapreechaStephen D Bentley
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Murat BalabanBirgitta Henriques-Normark
Jul 12, 2014·PloS One·Anne L WyllieKrzysztof Trzciński
Nov 20, 2014·Nature Communications·Nicholas J CroucherWilliam P Hanage
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Daniel StraumeLeiv Sigve Håvarstein
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Cheryl P Andam, William P Hanage
Jan 3, 2015·Science·Sandrine BorgeaudMelanie Blokesch
Feb 25, 2015·Frontiers in Microbiology·Scott T ChanceyDavid S Stephens
Nov 29, 2015·Trends in Microbiology·Noémie Matthey, Melanie Blokesch

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Citations

Feb 19, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ravinder KaurMichael E Pichichero
Nov 5, 2019·Frontiers in Microbiology·Uriel A Angulo-ZamudioNidia León-Sicairos
Mar 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·Laura Ledesma-GarciaPascal Hols
Nov 15, 2018·Frontiers in Microbiology·N Luisa Hiller, Raquel Sá-Leão
May 21, 2019·Frontiers in Microbiology·Max R SchroederDavid S Stephens
Nov 19, 2018·Journal of Hazardous Materials·Han HaoMin Jin
May 16, 2021·Applied and Environmental Microbiology·Luyao MaXiaonan Lu

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Methods Mentioned

BETA
confocal microscopy
PCR
PCRs

Software Mentioned

ImageJ
Rad CFX Manager
Imaris
Bio
Lasergene
CFX
SigmaPlot

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