A mechanism regulating the onset of Sox2 expression in the embryonic neural plate

PLoS Biology
Costis PapanayotouClaudio D Stern

Abstract

In vertebrate embryos, the earliest definitive marker for the neural plate, which will give rise to the entire central nervous system, is the transcription factor Sox2. Although some of the extracellular signals that regulate neural plate fate have been identified, we know very little about the mechanisms controlling Sox2 expression and thus neural plate identity. Here, we use electroporation for gain- and loss-of-function in the chick embryo, in combination with bimolecular fluorescence complementation, two-hybrid screens, chromatin immunoprecipitation, and reporter assays to study protein interactions that regulate expression of N2, the earliest enhancer of Sox2 to be activated and which directs expression to the largest part of the neural plate. We show that interactions between three coiled-coil domain proteins (ERNI, Geminin, and BERT), the heterochromatin proteins HP1alpha and HP1gamma acting as repressors, and the chromatin-remodeling enzyme Brm acting as activator control the N2 enhancer. We propose that this mechanism regulates the timing of Sox2 expression as part of the process of establishing neural plate identity.

References

Jul 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M RexP J Scotting
Mar 30, 1999·Mechanisms of Development·J SchofieldP Brickell
Feb 5, 2000·Current Biology : CB·J F Smothers, S Henikoff
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M MatsuiY Sasai
Aug 25, 2000·Molecular and Cellular Biology·G WangP B Singh
Mar 22, 2001·Molecular and Cellular Biology·J F Smothers, S Henikoff
Sep 22, 2001·The EMBO Journal·S A JacobsS Khorasanizadeh
Dec 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J J BainterK L Kroll
Sep 11, 2002·Developmental Biology·Andrea Streit
Jan 7, 2003·Genes & Development·Ariel A AvilionRobin Lovell-Badge
Sep 2, 2003·Neuron·Victoria GrahamLarysa Pevny
Oct 1, 2003·Nature Neuroscience·Magdalena BylundJonas Muhr
Jun 30, 2004·Developmental Biology·Gerhard Schlosser, Katja Ahrens
Sep 18, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jeremy B A GreenLance A Davidson
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Edward M De Robertis, Hiroki Kuroda
Oct 29, 2004·The EMBO Journal·Jonathan BaxterAmanda G Fisher
Dec 14, 2004·Development·Emilie DelauneLaurent Kodjabachian
Apr 15, 2005·Development·Claudio D Stern
May 5, 2005·Molecular and Cellular Biology·Ivana L de la SernaAnthony N Imbalzano
Jul 19, 2005·Genes & Development·Seongjin SeoKristen L Kroll
Jan 21, 2006·The EMBO Journal·Yasuyuki OhkawaAnthony N Imbalzano
Dec 5, 2006·Developmental Biology·Amy L DonnerRichard L Maas

❮ Previous
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Citations

Nov 26, 2014·Nature Reviews. Immunology·Nicolas BuchonSara Cherry
Oct 16, 2010·PloS One·Hong YuBalaji Polepalli Ramesh
May 6, 2011·The Journal of Biological Chemistry·Dafni-Eleutheria PefaniZoi Lygerou
Feb 18, 2012·Stem Cells and Development·Lisa S D Emmett, K Sue O'Shea
Sep 26, 2013·The Journal of Biological Chemistry·Christophe CaillatAnastassis Perrakis
Sep 27, 2013·PloS One·Golnaz A TabriziMichael Kessel
Apr 13, 2012·Nucleic Acids Research·J MarianiS K Nicolis
Jul 10, 2010·Science·John JaenikeSteve J Perlman
Oct 2, 2012·Briefings in Functional Genomics·Luis Teixeira
Apr 30, 2008·FEBS Letters·Ann-Kristin Ostlund Farrants
Jul 21, 2015·Genes·Virlana M ShchukaJennifer A Mitchell
Sep 15, 2009·Birth Defects Research. Part C, Embryo Today : Reviews·Crystal D RogersElena S Casey
Jan 26, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hervé AcloqueBertrand Pain
Jul 28, 2010·Alcoholism, Clinical and Experimental Research·Ling LongYing Peng
Oct 29, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chengyang HuangNaihe Jing
May 7, 2011·EMBO Reports·Iñaki Iturbe-OrmaetxeScott L O' Neill
Jan 29, 2013·Genesis : the Journal of Genetics and Development·Mohsin A F KhanClaudio D Stern
Jan 3, 2013·Development, Growth & Differentiation·Christian JeanBertrand Pain
Jun 18, 2011·Stem Cells·Magda SpellaStavros Taraviras
Jul 14, 2012·Developmental Biology·Timothy GrocottAndrea Streit
Nov 17, 2009·Developmental Biology·Andrea E WillsRichard M Harland
Jun 26, 2015·Nature Protocols·Sarah H Merkling, Ronald P van Rij
Dec 11, 2013·Developmental Biology·Andrew K Groves, Carole LaBonne
Oct 14, 2014·Frontiers in Physiology·Nikolaos MandalosEumorphia Remboutsika
Jan 4, 2017·Nature Reviews. Immunology·Miguel P SoaresLuis F Moita
Dec 7, 2016·Developmental Biology·Irene De AlmeidaClaudio D Stern
Apr 22, 2016·Molecular Biology of the Cell·Santiago O BouzasPablo H Strobl-Mazzulla
May 16, 2013·Nature Communications·Costis PapanayotouClaudio D Stern

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Datasets Mentioned

BETA
EU118175

Methods Mentioned

BETA
coimmunoprecipitation
two-hybrid
ChIP
immunoprecipitation
transfection
PCR

Software Mentioned

BiFCo

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