Abstract
This study is aimed at validating the hypothesis that administration of cyclosporine-A (CsA) would be protective in lung ischemia-reperfusion (I/R) injury and in exploring the underlying mechanism. Rabbits were divided into 4 groups: the control, sham operation, I/R, and I/R with CsA treatment. Flow cytometry was used to measure the mitochondrial membrane potential. Laser scanning confocal microscope was used to analyze mitochondrion permeability transition pore (MPTP). The apoptotic cell was detected by the TUNEL staining. Western blot was performed to analyze the protein expression levels. CsA not only attenuated the histopathologic alterations in lung and mitochondria after I/R injury, but also attenuated I/R injury through increasing MPP and inhibiting MPTP opening. Besides, CsA attenuated I/R injury through suppressing the release of cytochrome-c (CytC), inhibiting cell apoptosis and decreasing the expression levels of cyclophilin-D (Cyp-D), adenine nucleotide translocase 1 (ANT1) and voltage-dependent anion channel 1 (VDAC1). Finally, we found that Cyp-D knockdown inhibits I/R injury-induced MPTP opening and cell apoptosis. Our study found that the protective role of CsA on lung I/R injury depends on the inhibition of MPT...Continue Reading
References
Mar 1, 1986·Clinical Pharmacokinetics·R J PtachcinskiG J Burckart
Oct 8, 1998·The Journal of Cell Biology·R EskesJ C Martinou
Jan 5, 1999·European Journal of Biochemistry·M CromptonJ M Ward
Feb 4, 1999·Biophysical Journal·V PetronilliF Di Lisa
Mar 23, 2000·Cardiovascular Research·C Depre, H Taegtmeyer
Jan 19, 2002·IUBMB Life·E Blachly-Dyson, M Forte
May 3, 2002·The Journal of Thoracic and Cardiovascular Surgery·B KrishnadasanM S Mulligan
Sep 24, 2004·Lancet·Deirdre KellyRaymond Reding
Apr 1, 2005·Nature·Christopher P BainesJeffery D Molkentin
Sep 27, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Enrico LugliAndrea Cossarizza
May 29, 2007·Heart Failure Reviews·Antonio Rodríguez-SinovasDavid Garcia-Dorado
Aug 2, 2008·The New England Journal of Medicine·Christophe PiotMichel Ovize
Dec 9, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Radha AnanthakrishnanRavichandran Ramasamy
Feb 27, 2009·Basic Research in Cardiology·Christopher P Baines
Aug 18, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Marisol Ruiz-MeanaDavid Garcia-Dorado
Aug 18, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Giuseppe PetrosilloGiuseppe Paradies
Dec 20, 2014·Genetics and Molecular Research : GMR·T SongJ G Deng
Jan 23, 2015·Cell Death & Disease·H-Y LiZ-M Ye
Feb 11, 2016·Scientific Reports·Gentaro IkedaKensuke Egashira
Citations
Oct 3, 2019·Neurocritical Care·Xiang ZhouZhiTian Tan
Mar 5, 2020·Proteomics·Venkateswaran NatarajanKarthik Mallilankaraman
Jul 18, 2020·The Journal of International Medical Research·Haibo Zou, Xiaofeng Sun
Jul 9, 2020·Biomolecules·Massimo BonoraPaolo Pinton
Dec 19, 2018·Biomolecules·George A Porter, Gisela Beutner
Jun 4, 2020·Critical Care : the Official Journal of the Critical Care Forum·Martin CourLaurent Argaud
Jul 30, 2020·Free Radical Biology & Medicine·Nathalie TomboJean C Bopassa
May 23, 2021·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Hailey M ShepherdDaniel Kreisel
Jul 8, 2021·Translational Vision Science & Technology·Marco H JiEdward H Wood