Apr 10, 2020

Comparative secretome of Magnaporthe oryzae identified proteins involved in virulence and cell wall integrity

BioRxiv : the Preprint Server for Biology
N. LiuXunli Lu


Plant fungal pathogens secrete numerous proteins into the apoplast at the plant minus fungus contact sites to facilitate colonization. Only a few secreted proteins were functionally characterized in Magnaporthe oryzae, the fungal pathogen causing rice blast disease worldwide. ALG3 is an lower case Greek alphaminus1, 3minusmannosyltransferase function in Nminusglycan synthesis for secreted N-glycosylated proteins, and the {triangleup}alg3 mutants show strong defects in cell wall integrity and fungal virulence, indicating a potential effect on the secretion of multiple proteins. In this study, we compared the secretome of wild type and {triangleup}alg3 mutants, and identified 51 proteins that require ALG3 for proper secretion. These are predicted to be involved in metabolic processes, interspecies interactions, cell wall organization, and response to chemicals. The tested secreted proteins localized at the apoplast region surrounding the fungal infection hyphae. Moreover, the Nminusglycosylation of candidate proteins was significantly changed in the {triangleup}alg3 mutant, leading to the reduction of protein secretion and abnormal protein localization. Furthermore, we tested the function of two genes, one is a previously reporte...Continue Reading

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