PMID: 2123173Sep 1, 1990

A medium for the isolation, enumeration and rapid presumptive identification of injured Clostridium perfringens and Bacillus cereus

The Journal of Applied Bacteriology
A M HoodC R Dane

Abstract

A blood-free egg yolk medium (BCP) containing pyruvate, inositol, mannitol and a bromocresol purple indicator in a nutrient agar base has been developed to initiate the growth of Clostridium perfringens. It is comparable to blood agar for the growth of normal, chilled stored vegetative cells and heat-injured spores of Cl. perfringens and Bacillus cereus. It has the advantage over blood agar in exhibiting presumptive evidence of Cl. perfringens (production of lecithinase and inositol fermentation) after an overnight incubation at 43 degrees - 45 degrees C. Pyruvate, catalase and other hydrogen peroxide degraders were found to remove toxins rapidly formed in media exposed to air and light. Free radical scavengers of superoxide, hydroxyl ions and singlet oxygen were ineffective. Without scavengers the formation of 10-20 micrograms/ml hydrogen peroxide in the exposed medium was indicated and found lethal to injured Cl. perfringens. The BCP medium has been used successfully for the rapid identification and enumeration of Cl. perfringens in foods and faeces from food poisoning outbreaks and cases of suspected infectious diarrhoea. Greater recovery of severely injured vegetative Cl. perfrigens could be obtained by pre-incubation at 37...Continue Reading

References

Aug 1, 1978·Applied and Environmental Microbiology·J CarlssonJ Wrethén
Feb 1, 1976·Journal of Bacteriology·R S Flowers, D M Adams
Sep 1, 1976·Applied and Environmental Microbiology·S M Harmon, D A Kautter
Jul 1, 1977·Canadian Journal of Microbiology·A H HauschildR Vahlefeld
Aug 1, 1978·Applied and Environmental Microbiology·R D RolfeJ T Barrett
Jul 1, 1976·Applied and Environmental Microbiology·M S ChowdhuryH S Levinson
Oct 1, 1976·Archives of Biochemistry and Biophysics·H Misra, I Fridovich
Apr 1, 1977·Applied and Environmental Microbiology·S M Harmon, D A Kautter
Dec 1, 1974·The Journal of Applied Bacteriology·G A Prentice, L F Clegg
Dec 1, 1974·The Journal of Applied Bacteriology·P M Handford
Mar 1, 1968·Journal of General Microbiology·J Farrell, A H Rose
May 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J M SteimR L Rader
May 18, 1965·Biochemical and Biophysical Research Communications·K Ring
Oct 1, 1980·Archives of Biochemistry and Biophysics·S Kong, A J Davison
Feb 1, 1982·Applied and Environmental Microbiology·P J PadgettN R Krieg
Jul 1, 1978·Journal of Food Protection·Harriet Rappaport, J M Goepfert
Jun 1, 1982·Journal of Food Protection·R G Labbé, Kirk E Norris

Citations

Oct 1, 1992·International Journal of Food Microbiology·P van Netten, J M Kramer
Oct 1, 1995·International Journal of Food Microbiology·G H WeenkD A Mossel
Dec 1, 1996·International Journal of Food Microbiology·S M CzechowiczE A Zottola
Dec 1, 1998·Journal of Applied Microbiology·D P Sartory, J Watkins
Jan 5, 2008·Prikladnaia biokhimiia i mikrobiologiia·A L Briukhanov, A I Netrusov
Dec 18, 2014·Letters in Applied Microbiology·J WatkinsUK Standing Committee of Analysts
Nov 26, 2013·Journal of Applied Microbiology·M AbdelmassihJ Mahillon
Sep 28, 2007·Letters in Applied Microbiology·M H Farris, J B Olson
May 9, 1998·Applied and Environmental Microbiology·V Mäntynen, K Lindström

Related Concepts

Bacillus cereus
Clostridium perfringens
Diarrhea
Disease Outbreaks
Feces
Food Microbiology
Foodborne Disease
Free Radicals
Oxydol
Spores, Bacterial

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