A member of the phylogenetically conserved CAD family of transcriptional regulators is dramatically up-regulated during the programmed cell death of skeletal muscle in the tobacco hawkmoth Manduca sexta

Developmental Biology
D SunL M Schwartz

Abstract

The intersegmental muscles (ISMs) of the tobacco hawkmoth Manduca sexta participate in the emergence behavior of the adult moth at the end of metamorphosis and then die during the subsequent 30-hr period. The trigger for this death is a decline in the circulating titer of the insect molting hormone 20-hydroxyecdysone (20-HE). Previous work has demonstrated that the ability of the ISMs to die is dependent on new gene expression. Using a differential hybridization cloning strategy, a cDNA library made from the ISMs committed to die was screened, and four up-regulated clones were isolated. One clone, 18-56, was selected for this study. Northern and Western analysis demonstrated that while clone 18-56 was expressed in all tissues examined and during every stage of ISM development, there was a dramatic increase in expression at both mRNA and protein levels when the ISMs became committed to die. If ISM death was delayed by an injection of 20-HE on the day proceeding adult emergence, 18-56 expression remained at basal levels. Immunocytochemistry demonstrated that 18-56 protein was located predominantly in nuclei prior to the commitment of the ISMs to die and then accumulated to high levels in cytoplasm at the time of cell death. DNA s...Continue Reading

Citations

Oct 23, 1997·Protein Science : a Publication of the Protein Society·A Beyer
Jun 16, 2001·Cell Biology International·M E Jones, L M Schwartz
May 17, 2008·Cell Death and Differentiation·L M Schwartz
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y HuL M Schwartz
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Nov 23, 2006·Journal of Insect Physiology·Rush H OliverTimothy A Mousseau
Jan 21, 2010·The Biochemical Journal·Yatender KumarGhanshyam Swarup
May 9, 2002·American Journal of Physiology. Cell Physiology·Lawrence M Schwartz, Robert L Ruff
Nov 14, 1998·Biochemical and Biophysical Research Communications·K HolzmannG Sauermann
Oct 15, 2021·The Journal of Experimental Biology·David S BrooksErika R Geisbrecht

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