A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

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Peisen SuLingrang Kong

Abstract

Background: Salt and drought are the main abiotic stresses that restrict yield of crops. It is reported that peroxidases (PRXs) are involved in various abiotic stress responses. However, few wheat PRXs are characterized in the mechanism of abiotic stresses. Results: In this study, a novel wheat peroxidase (PRX) gene named TaPRX-2A, a member of wheat class III peroxidase gene family, was cloned and characterized in salt stress response. Based on the identification and evolutionary analysis of class III PRXs in 12 plants, we proposed an evolutionary model that TaPRX-2A might have experienced some exon fusion events during evolution. We also detected the positive selection of PRX domain in 13 PRXs involving our evolutionary model, and found 2 or 6 positively selected sites during TaPRX-2A evolution. The results of expression pattern showed that TaPRX-2A exhibited relatively higher expression levels in root tissue compared with that of leaf and stem tissues by using qRT-PCR. This TaPRX-2A was also induced by some stresses and hormone treatments including PEG6000, NaCl, hydrogen peroxide (H 2 O 2 ), salicylic acid (SA), methyljasmonic acid (MeJA) and abscisic acid (ABA). Transgenic wheat plants with overexpression of TaPRX-2A showed...Continue Reading

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