PMID: 9555902Apr 29, 1998Paper

A membrane-associated protein, FliX, is required for an early step in Caulobacter flagellar assembly

Journal of Bacteriology
C D MohrLucy Shapiro

Abstract

The ordered assembly of the Caulobacter crescentus flagellum is accomplished in part through the organization of the flagellar structural genes in a regulatory hierarchy of four classes. Class II genes are the earliest to be expressed and are activated at a specific time in the cell cycle by the CtrA response regulator. In order to identify gene products required for early events in flagellar assembly, we used the known phenotypes of class II mutants to identify new class II flagellar genes. In this report we describe the isolation and characterization of a flagellar gene, fliX. A fliX null mutant is nonmotile, lacks a flagellum, and exhibits a marked cell division defect. Epistasis experiments placed fliX within class II of the flagellar regulatory hierarchy, suggesting that FliX functions at an early stage in flagellar assembly. The fliX gene encodes a 15-kDa protein with a putative N-terminal signal sequence. Expression of fliX is under cell cycle control, with transcription beginning relatively early in the cell cycle and peaking in Caulobacter predivisional cells. Full expression of fliX was found to be dependent on ctrA, and DNase I footprinting analysis demonstrated a direct interaction between CtrA and the fliX promoter...Continue Reading

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Citations

Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Patrick D Curtis, Yves V Brun
Aug 6, 2015·PLoS Genetics·Andrew M PhillipsDaniel B Kearns
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael T LaubHarley H McAdams
Oct 7, 2003·Annual Review of Microbiology·Nora Ausmees, Christine Jacobs-Wagner
Feb 19, 2021·Developmental Cell·Manisha SiwachSunish Kumar Radhakrishnan
Sep 28, 1999·Journal of Bacteriology·E K ManganJ W Gober
May 7, 2009·Genes & Development·Godefroid CharbonChristine Jacobs-Wagner

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