A membrane-bound nitrate reductase encoded by the narGHJI operon is responsible for anaerobic respiration in Halomonas maura.

Extremophiles : Life Under Extreme Conditions
Montserrat ArgandoñaAna del Moral

Abstract

The halophilic bacterium Halomonas maura is capable of anaerobic respiration on nitrates. By insertional mutagenesis with the minitransposon Tn-5 we obtained the mutant Tc62, which was incapable of anaerobic respiration on nitrates. An analysis of the regions adjacent to the transposon allowed us to characterize the membrane-bound anaerobic-respiratory nitrate reductase narGHJI gene cluster in H. maura. We identified consensus sequences for fumarate and nitrate reductase regulator (FNR)-like protein-binding sites in the promoter regions of the nar genes and consensus sequences corresponding to the NarL binding sites upstream of the nar genes. RT-PCR analysis showed that the narGHJI operon was expressed in response to anaerobic conditions when nitrate was available as electron acceptor. This membrane-bound nitrate reductase is the only enzyme responsible for anaerobic respiration on nitrate in H. maura. In this article we discuss the possible relationship between this enzyme and a dissimilatory nitrate-reduction-to-ammonia process (DNRA) in H. maura and its role in the colonization of the rhizosphere.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1991·Archives of Biochemistry and Biophysics·D HernandezJ J Rowe
Jun 1, 1991·Journal of Bacteriology·J A DeMoss, P Y Hsu
Sep 1, 1989·Journal of General Microbiology·K J DaviesL Boddy
Sep 1, 1989·Applied and Environmental Microbiology·J J NietoF Ruiz-Berraquero
Aug 1, 1995·Applied and Environmental Microbiology·J P CarterD J Richardson
Jan 1, 1994·Antonie van Leeuwenhoek·S Spiro
Jan 1, 1993·Archives of Microbiology·W G Zumft
Jan 1, 1993·Trends in Biochemical Sciences·M D Marger, M H Saier
Jun 30, 1996·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T OshimaT Horiuchi
Jun 29, 1999·Journal of Bacteriology·R J Van SpanningN Saunders
Jul 9, 1999·Biochimica Et Biophysica Acta·L Philippot, O Højberg
Mar 21, 2000·Research in Microbiology·I LlamasA del Moral
May 4, 2000·Systematic and Applied Microbiology·M R MormileB M Peyton
Apr 6, 2001·Cellular and Molecular Life Sciences : CMLS·D J RichardsonC J Taylor
Apr 6, 2001·Cellular and Molecular Life Sciences : CMLS·J W Moir, N J Wood
Oct 12, 2001·International Journal of Systematic and Evolutionary Microbiology·S BouchotrochV Béjar
Oct 3, 2002·Biochimica Et Biophysica Acta·Laurent Philippot
Apr 20, 2006·Antonie van Leeuwenhoek·Inmaculada LlamasEmilia Quesada
Oct 1, 1993·Trends in Ecology & Evolution·D Lloyd

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