A membrane transporter for tryptophan composed of RNA

RNA
Teresa JanasM Yarus

Abstract

We have incorporated an RNA binding site for the biological amino acid tryptophan within an RNA complex with affinity for phospholipid bilayer membranes. The resulting RNA (9:10Trp) creates a selective route through the bilayer for the amino acid. Binding and enhanced tryptophan permeability are nonlinear in RNA concentration, suggesting that RNA aggregation is required for both. Tryptophan permeability saturates with increased concentration, though at approximately 1000-fold greater level than when binding a free aptamer. The RNA (9:10Trp) complex, bound at a mean of two per liposome, halves the activation energy for tryptophan transport (to 46 kJ/mole), specifically increasing tryptophan entry to a maximal velocity of 0.5 sec(-1) per liposome with little or no accompanying increase in general permeability. Individual RNAs turn over tens of thousands of times at high tryptophan concentration. Thus, a specific passive membrane transporter whose properties overlap those of single-molecule transporter proteins, can be made of RNA alone. Permeability changes probably rely on disturbances in lipid conformation as well as on an advantageous low free energy position for tryptophan at the membrane. Other RNA activities may yield other...Continue Reading

References

Jul 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E N NjagiD J Pearson
Dec 24, 1991·Biochemistry·J C McIntyre, R G Sleight
Jun 18, 1985·Biochemistry·H EllensF C Szoka
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·O SandreF Brochard-Wyart
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·A KhvorovaM Yarus
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·A VlassovM Yarus
Dec 12, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·R M RaphaelB Zeks
Nov 14, 2002·Annual Review of Genetics·Michael Yarus
Feb 21, 2003·Astrobiology·David DeamerLouis J Allamandola
Oct 17, 2003·RNA·Tadeusz Janas, Michael Yarus

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Citations

Nov 1, 2013·Chemical Reviews·Kepa Ruiz-MirazoAndrés de la Escosura
Sep 28, 2005·Nucleic Acids Research·Irene Majerfeld, Michael Yarus
Apr 28, 2006·Nucleic Acids Research·Tadeusz JanasMichael Yarus
May 3, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Eörs Szathmáry
Sep 1, 2007·HFSP Journal·Patrick Forterre, Simonetta Gribaldo
Aug 4, 2010·Cold Spring Harbor Perspectives in Biology·Sheref S Mansy
Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Itay Budin, Jack W Szostak
Mar 16, 2007·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Jacquelyn A Thomas, F R Rana
Jan 30, 2008·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Matti Jalasvuori, Jaana K H Bamford
Apr 29, 2006·FEBS Letters·Janina GabrielskaWiesław I Gruszecki
May 6, 2015·FEBS Letters·Teresa JanasTadeusz Janas
Dec 19, 2009·Chemistry and Physics of Lipids·Teresa JanasTadeusz Janas
Mar 5, 2015·Annals of the New York Academy of Sciences·Ádám KunEörs Szathmáry
Jun 30, 2010·Cellular & Molecular Biology Letters·Teresa Janas, Tadeusz Janas
Dec 3, 2013·Cellular & Molecular Biology Letters·Krzysztof NowotarskiTeresa Janas
May 20, 2015·Journal of Theoretical Biology·Andrés de la EscosuraKepa Ruiz-Mirazo
Sep 20, 2011·Biochimica Et Biophysica Acta·Teresa Janas, Tadeusz Janas
May 28, 2011·Biochimica Et Biophysica Acta·Teresa JanasTadeusz Janas
Jul 14, 2005·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Alexander Vlassov
Jan 16, 2019·International Journal of Molecular Sciences·Teresa JanasTadeusz Janas
Feb 26, 2020·Epigenomics·Javid Sadri NahandMichael R Hamblin
Nov 25, 2018·Journal of Theoretical Biology·Zsuzsanna Vig-MilkovicsEörs Szathmáry
Jan 11, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Tadeusz JanasMichael Yarus
Jul 25, 2018·Biochimica Et Biophysica Acta. Biomembranes·Karolina SapońTadeusz Janas
Nov 18, 2020·International Journal of Molecular Sciences·Teresa JanasTadeusz Janas
Jun 1, 2021·Frontiers in Cell and Developmental Biology·Margarida BeatrizCarla Lopes
Dec 8, 2006·Genome Research·Anthony C Forster, George M Church

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