A mesenchymal perspective of Müllerian duct differentiation and regression in Amhr2-lacZ mice.

Molecular Reproduction and Development
Nelson A ArangoRichard R Behringer

Abstract

The Müllerian ducts give rise to the female reproductive tract, including the Fallopian tubes, uterus, cervix, and anterior vagina. In male embryos, the Müllerian ducts regress, preventing the formation of female organs. We introduced the bacterial lacZ gene, encoding beta-galactosidase (beta-gal), into the AMHR-II locus (Amhr2) by gene targeting in mouse embryonic stem (ES) cells to mark Müllerian duct differentiation and regression. We show that Amhr2-lacZ heterozygotes express beta-gal activity in an Amhr2-specific pattern. In the gonads, beta-gal activity was detected in Sertoli cells of the testes from 2 weeks after birth, and fetal ovaries and granulosa cells of the adult ovary. beta-gal activity was first detected in the rostral mesenchyme of the Müllerian ducts at 12.5 days post coitus (dpc) in both sexes but soon thereafter expression was found along the entire length of the Müllerian ducts with higher levels initially found in males. In females, beta-gal activity was restricted to one side of the ductal mesoepithelium, whereas in males beta-gal expression encircled the duct. beta-gal activity was also detected in the coelomic epithelium at 13.5 and 14.5 dpc. In male embryos, mesenchymal beta-gal activity permitted the...Continue Reading

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Citations

Sep 2, 2010·Proceedings of the National Academy of Sciences of the United States of America·Pradeep S TanwarJose M Teixeira
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jaeyeon KimMartin M Matzuk
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Mar 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jitu W GeorgeJose M Teixeira

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