PMID: 8614121May 1, 1996Paper

A meta-analytic evaluation of the polymerase chain reaction for the diagnosis of HIV infection in infants

JAMA : the Journal of the American Medical Association
D K OwensS Sonnad

Abstract

To evaluate the sensitivity and specificity of the polymerase chain reaction (PCR) for the diagnosis of infection with human immunodeficiency virus (HIV) in infants. We used studies published between 1988 and 1994 identified in a literature search of 17 databases, including MEDLINE. Studies were included if DNA amplification by PCR was performed on peripheral blood mononuclear cells from infants or children. Two investigators independently extracted data. The study design was assessed independently by 2 investigators who were blinded to study results. Thirty-two studies met the inclusion criteria and were analyzed. The median reported sensitivity was 91.6% (range, 31%-100%), and the median specificity was 100% (range, 50%-100%). A summary receiver operating characteristic curve based on all 32 studies indicated that PCR has a maximum joint sensitivity and specificity between 93.2% and 94.9%. Subgroup analysis indicated that the joint sensitivity and specificity was significantly (P = .04) higher in older infants (98.2%) than in neonates (aged < or = 30 days; 93.3%). For infants at low risk of perinatal transmission (probability of transmission, 8.3%), the positive predictive value for PCR is 55.8% in neonates and 83.2% in older...Continue Reading

Citations

Aug 7, 2004·Journal of Clinical Microbiology·Chung-Chen LiLisa M Frenkel
Jul 11, 2008·Journal of Clinical Microbiology·Caroline MitchellUNKNOWN Dried Blood Spot Working Group of the Infant Maternal Pediatric Adolescent AIDS Clinical Trials Network
Oct 9, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·E G Hermione Lyall
Mar 21, 2012·BMC Research Notes·Steven BaveewoRhoda K Wanyenze
Jan 5, 2002·Biometrics·R Balasubramanian, S W Lagakos
Jan 9, 1998·The Journal of Hospital Infection·J Gray
Sep 25, 2010·Journal of Virological Methods·Marco Rossi de GasperisFrancesco Callea
Feb 20, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Peter Rohner, Raymond Auckenthaler
Sep 25, 2002·Clinics in Laboratory Medicine·Paul Palumbo
Mar 4, 2000·Pediatric Clinics of North America·K Nielsen, Y J Bryson
Jul 1, 1997·Medical Decision Making : an International Journal of the Society for Medical Decision Making·D K OwensR F Nease
Sep 16, 1998·AIDS Research and Human Retroviruses·L WaltherR Thorstensson
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·A J De BaetsK Fransen

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