Abstract
To investigate the bioactive metabolite of endophytic fungus from Kalimeris indica. A strain ZJLQ336 was separated from the leaves of K. indica. It was identified as Cadophora orchidicola based on the phylogenetic analysis of ITS-rDNA sequences. From the fermentation broth a metabolite cercosporamide (compound 1) was isolated, and its structure was determined by spectroscopic analysis. Additionally, this compound was subjected to bioactivity assays, including antifungal activity against seven plant pathogenic fungi, as well as its potential immunoregulatory effects on HEK-BLUE™-hTLR4 cells, splenocytes and macrophages. The results showed that cercosporamide had strong growth inhibition against five common plant pathogenic fungi, including Pestalotia diospyri, Botrytis cinerea, Fusarium oxysporum, Sclerotium rolfsii and Penicillum digitatum with EC50 values of 5·29 × 10-3 , 0·61, 0·93, 2·89 and 6·7 μg ml-1 respectively. Among which S. rolfsii was one of the main pathogens in K. indica. Moreover, cercosporamide not only significantly stimulated TLR4 activation, splenocyte proliferation and production of cytokines, IFN-γ and TNF-α, but also up-regulated the production of TNF-α and NO in RAW264.7 macrophages clearly. This is the fi...Continue Reading
References
Aug 2, 2003·The Journal of Biological Chemistry·Shizuo Akira
Feb 28, 2004·Journal of Natural Products·Gary StrobelJames Harper
Aug 11, 2004·Eukaryotic Cell·Andrea SussmanXiang S Ye
Aug 9, 2007·Mycologia·Fumiaki Usuki, Kazuhiko Narisawa
Sep 11, 2008·Genesis : the Journal of Genetics and Development·Dmitry A Ovchinnikov
Aug 18, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Yusuke SatoDave S B Hoon
Jan 15, 2011·Cancer Research·Bruce W KonicekJeremy R Graff
Jan 20, 2011·The New Phytologist·K K Newsham
Aug 5, 2011·Applied Microbiology and Biotechnology·Li-Wei WangChristian P Kubicek
Nov 10, 2011·Mycologia·Melissa J DayRandolph S Currah
May 1, 2012·European Journal of Pharmacology·Fengyang ChenShifang Xu
Jul 31, 2012·Chemistry & Biology·Souvik KusariMichael Spiteller
Aug 11, 2012·The Journal of Antimicrobial Chemotherapy·Anupam AdhikariSubrata Majumdar
Feb 26, 2013·Molecular Neurobiology·Kurt Lucas, Michael Maes
Mar 19, 2013·Chemical Society Reviews·Xiaohui WangHang Yin
Apr 16, 2013·Chemical Reviews·Jin-Ming GaoJian-Chun Qin
May 3, 2013·PloS One·Zhen-Zhu SuChu-Long Zhang
Jun 28, 2013·BMC Plant Biology·Sameh S M SolimanManish N Raizada
Apr 3, 2014·Journal of Agricultural and Food Chemistry·Jian XiaoJin-Ming Gao
Jun 15, 2014·World Journal of Microbiology & Biotechnology·Jiaying WangLiwei Wang
Dec 4, 2014·Cell Reports·Christos G GkogkasNahum Sonenberg
Dec 6, 2014·European Journal of Immunology·Mateja Manček-Keber, Roman Jerala
Feb 11, 2015·Carbohydrate Polymers·Hongxiang SunHui Wang
Feb 24, 2015·Journal of Ethnopharmacology·Rui-Feng ZhongShang-Gao Liao
Mar 21, 2015·Biotechnology Letters·Jutta Ludwig-Müller
May 11, 2015·Mycorrhiza·Martin VohníkRadka Sudová
Jun 9, 2015·Frontiers in Chemistry·David J Newman, Gordon M Cragg
Dec 1, 2015·Fungal Biology·Jing LuoNing Zhang
Feb 2, 2016·Microbes and Infection·Javier MegíasM Luisa Gil
Feb 13, 2016·Natural Product Reports·Marion MillotSophie Tomasi
May 18, 2016·Journal of Natural Products·Xuelong YuXingyun Chai
Jan 11, 2018·Journal of Cellular Physiology·Abbas Shapouri-MoghaddamAmirhossein Sahebkar