A metabonomic analysis on the response of Enterobacter cloacae from coastal outfall for land-based pollutant under phoxim stress

Archives of Microbiology
Dijun ZhangXiurong Su

Abstract

Enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. The secretion of extended-spectrum β-lactamases (ESBLs) and cephalosporinase (AmpC) endows E. cloacae with strong drug resistance. In a previous study by our group, protein expression of E. cloacae under phoxim stress was measured by two-dimensional electrophoresis. Here, nuclear magnetic resonance was used to detect differences in E. cloacae metabonomics when under phoxim stress. We determined that there are 29 types of metabolites that differ between phoxim stress and normal culture conditions. Among these, 6 types of metabolites were upregulated in the phoxim stress group, and 23 types of metabolites were inhibited. Though enrichment analysis, seven pathways were identified by different expression levels of metabolites, which were involved in DNA and RNA synthesis, DNA damage repair, antioxidation and functions of the cell membrane and cell wall. The mechanism underlying how phoxim affects E. cloacae was determined by studying the results of both two-dimensional electrophoresis in our prior work and the analysis of E. cloacae metabonomic changes under phoxim stress.

References

Aug 1, 1990·Journal of Bacteriology·I R Tsaneva, B Weiss
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J T GreenbergB Demple
Dec 30, 1999·Journal of Bacteriology·P J Pomposiello, B Demple
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·G S AttardS Jackowski
Oct 18, 2000·Toxicology and Applied Pharmacology·K CarlsonM Ehrich
Aug 21, 2007·Lancet·Michael EddlestonAndrew H Dawson
Apr 18, 2008·Annals of Nutrition & Metabolism·Andrea Caroline KnappStephan Krähenbühl
May 4, 2012·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Dec 22, 2012·Acta Biochimica Et Biophysica Sinica·Xiaoling Li
Jan 15, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Antonio CascioSalvatore Giordano
Jun 15, 2014·Analytica Chimica Acta·Helene FaberUwe Karst
Sep 1, 2015·Archives of Biochemistry and Biophysics·Huihui FuHaichun Gao

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Citations

Sep 3, 2017·Environmental Science and Pollution Research International·Chundan ZhangDewen Ding

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Methods Mentioned

BETA
electrophoresis
nuclear magnetic resonance
NMR
PCA
electrophoreses

Software Mentioned

MetaboAnalyst
Chenomx NMR Suite

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