A metagenomic window into carbon metabolism at 3 km depth in Precambrian continental crust

The ISME Journal
Cara MagnaboscoTullis C Onstott

Abstract

Subsurface microbial communities comprise a significant fraction of the global prokaryotic biomass; however, the carbon metabolisms that support the deep biosphere have been relatively unexplored. In order to determine the predominant carbon metabolisms within a 3-km deep fracture fluid system accessed via the Tau Tona gold mine (Witwatersrand Basin, South Africa), metagenomic and thermodynamic analyses were combined. Within our system of study, the energy-conserving reductive acetyl-CoA (Wood-Ljungdahl) pathway was found to be the most abundant carbon fixation pathway identified in the metagenome. Carbon monoxide dehydrogenase genes that have the potential to participate in (1) both autotrophic and heterotrophic metabolisms through the reversible oxidization of CO and subsequent transfer of electrons for sulfate reduction, (2) direct utilization of H2 and (3) methanogenesis were identified. The most abundant members of the metagenome belonged to Euryarchaeota (22%) and Firmicutes (57%)-by far, the highest relative abundance of Euryarchaeota yet reported from deep fracture fluids in South Africa and one of only five Firmicutes-dominated deep fracture fluids identified in the region. Importantly, by combining the metagenomics da...Continue Reading

References

Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W B WhitmanW J Wiebe
Nov 28, 2001·Applied and Environmental Microbiology·K TakaiJ K Fredrickson
Nov 7, 2003·Applied and Environmental Microbiology·Volker Müller
Dec 3, 2003·Environmental Microbiology·T C OnstottR Wilson
Feb 10, 2004·Applied and Environmental Microbiology·K L LondryD J Des Marais
Sep 17, 2005·International Journal of Systematic and Evolutionary Microbiology·Sofiya N ParshinaAlfons J M Stams
Dec 8, 2005·Applied and Environmental Microbiology·Duane P MoserT C Onstott
Jun 1, 1990·Applied and Environmental Microbiology·F H Chapelle, D R Lovley
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jennifer F BiddleKai-Uwe Hinrichs
Mar 4, 2006·Science·Francesca D CiccarelliPeer Bork
May 26, 1961·Science·H Craig
Dec 7, 2007·Biochemical and Biophysical Research Communications·Amy V CallaghanGerben J Zylstra
Oct 11, 2008·Science·Dylan ChivianTullis C Onstott
May 1, 2002·FEMS Microbiology Ecology·H T S Boschker, J J Middelburg
Jan 11, 2011·Applied and Environmental Microbiology·Ivan A Berg
Jun 4, 2011·Nature·G BorgonieT C Onstott
Jun 21, 2011·Bioinformatics·Yu PengFrancis Y L Chin
Jul 17, 2012·Bioinformatics·Doug HyattEdward C Uberbacher
Oct 13, 2012·Bioinformatics·Limin FuWeizhong Li
Apr 16, 2013·Applied and Environmental Microbiology·William J BrazeltonMatthew O Schrenk
Sep 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shino SuzukiKenneth H Nealson

❮ Previous
Next ❯

Citations

Oct 16, 2016·Nature Communications·Alexandre BagnoudRizlan Bernier-Latmani
Mar 4, 2017·Letters in Applied Microbiology·T Hoshino, F Inagaki
Dec 16, 2016·International Journal of Systematic and Evolutionary Microbiology·Olga A PodosokorskayaIlya V Kublanov
Jun 23, 2018·FEMS Microbiology Ecology·Margarita Lopez-FernandezMark Dopson
Aug 31, 2016·Nature Microbiology·Brett J BakerThijs J G Ettema
Jul 18, 2019·Environmental Microbiology·Magali Ranchou-PeyruseAnthony Ranchou-Peyruse
Nov 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Maggie C Y LauTullis C Onstott
Feb 23, 2017·Frontiers in Microbiology·Kaitlin R RempfertAlexis S Templeton
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Cristina EscuderoRicardo Amils
Jun 27, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sean McMahon, Magnus Ivarsson
Aug 6, 2019·Frontiers in Microbiology·Avishek DuttaSukanta Roy
Oct 6, 2020·Frontiers in Microbiology·Vitaly V KadnikovNikolai V Ravin
Mar 15, 2019·The ISME Journal·Amy R SmithFrederick S Colwell
Apr 8, 2021·The ISME Journal·Eric D BecraftRamunas Stepanauskas
Apr 17, 2021·Frontiers in Microbiology·Cody S SheikBrandy M Toner
May 12, 2021·Environmental Microbiology·Jose L SanzRicardo Amils

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.