A metastable rRNA junction essential for bacterial 30S biogenesis

Nucleic Acids Research
Indra Mani SharmaS A Woodson

Abstract

Tertiary sequence motifs encode interactions between RNA helices that create the three-dimensional structures of ribosomal subunits. A Right Angle motif at the junction between 16S helices 5 and 6 (J5/6) is universally conserved amongst small subunit rRNAs and forms a stable right angle in minimal RNAs. J5/6 does not form a right angle in the mature ribosome, suggesting that this motif encodes a metastable structure needed for ribosome biogenesis. In this study, J5/6 mutations block 30S ribosome assembly and 16S maturation in Escherichia coli. Folding assays and in-cell X-ray footprinting showed that J5/6 mutations favor an assembly intermediate of the 16S 5' domain and prevent formation of the central pseudoknot. Quantitative mass spectrometry revealed that mutant pre-30S ribosomes lack protein uS12 and are depleted in proteins uS5 and uS2. Together, these results show that impaired folding of the J5/6 right angle prevents the establishment of inter-domain interactions, resulting in global collapse of the 30S structure observed in electron micrographs of mutant pre-30S ribosomes. We propose that the J5/6 motif is part of a spine of RNA helices that switch conformation at distinct stages of assembly, linking peripheral domains ...Continue Reading

References

Jan 1, 1991·Annual Review of Biochemistry·H F Noller
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Powers, H F Noller
Nov 20, 1988·Journal of Molecular Biology·S SternH F Noller
Mar 20, 1988·Journal of Molecular Biology·S SternH F Noller
Jul 20, 1993·Journal of Molecular Biology·T PowersH F Noller
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·J C PaillartR Marquet
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·T AsaiC L Squires
Aug 9, 2002·RNA·Matthieu G Gagnon, Sergey V Steinberg
Nov 18, 2003·Journal of Bacteriology·Dmitry ZaporojetsCatherine L Squires
Jan 20, 2004·Nature Structural & Molecular Biology·Kristi L Holmes, Gloria M Culver
Dec 18, 2004·Science·Arkadiusz ChworosLuc Jaeger
Jun 14, 2005·Trends in Biochemical Sciences·Peter B Moore, Thomas A Steitz
Jul 19, 2005·Journal of Molecular Biology·Tadepalli AdilakshmiSarah A Woodson
Aug 4, 2005·Methods : a Companion to Methods in Enzymology·Elaine M Youngman, Rachel Green
Sep 6, 2005·Science·Harry F Noller
Dec 2, 2005·Nature·Megan W T TalkingtonJames R Williamson
Dec 24, 2005·RNA·Aurélie Lescoute, Eric Westhof
Oct 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Veysel BerkJamie H Doudna Cate
Nov 28, 2008·Nucleic Acids Research·Luc JaegerCody Geary
Apr 4, 2009·Nature Structural & Molecular Biology·Priya Ramaswamy, Sarah A Woodson
Mar 2, 2010·Journal of Molecular Biology·Anne E BunnerJames R Williamson
Jan 1, 2009·Methods in Enzymology·Tadepalli AdilakshmiSarah A Woodson
Oct 30, 2010·Science·Anke M MulderJames R Williamson
Aug 9, 2011·Journal of Molecular Biology·Megan MayerleSarah A Woodson
Jun 6, 2012·Nature Structural & Molecular Biology·Sergey MelnikovMarat Yusupov
Aug 3, 2012·Journal of the American Chemical Society·Kady-Ann SteenKevin M Weeks
Sep 25, 2012·Journal of Molecular Biology·Wade W GrabowLuc Jaeger
Feb 5, 2013·Wiley Interdisciplinary Reviews. RNA·Wade W GrabowLuc Jaeger
Jul 10, 2013·Wiley Interdisciplinary Reviews. RNA·Junya IshikawaYoshiya Ikawa
Feb 14, 2014·Nature·Hajin KimSarah A Woodson
May 27, 2014·Accounts of Chemical Research·Wade W Grabow, Luc Jaeger
Sep 10, 2014·Nature Structural & Molecular Biology·Neha Gupta, Gloria M Culver
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yi PengSarah A Woodson
Feb 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jennifer L McGinnisKevin M Weeks
Nov 10, 2016·EMBO Reports·Sergey MelnikovMarat Yusupov
Jun 6, 2017·Trends in Biochemical Sciences·Dieter KresslerJochen Baßler

❮ Previous
Next ❯

Methods Mentioned

BETA
footprinting
FRET
X-ray
transmission electron microscopy
electron microscopy

Software Mentioned

WINDAQ
ImageQuant
pSpur
Thermo Proteome Discoverer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.