PMID: 9433943Jan 20, 1998Paper

A method for canine nasal mucosa superfusion, in vivo; evidence that pseudomonas-induced neutrophil migration is inhibited by alpha2-adrenoceptor agonist

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
P StjärneJ A Nadel

Abstract

A method for isolating and superfusing a segment of the nasal cavity in the anaesthetized dog was developed. Introduction of Pseudomonas aeruginosa supernatant (PA), which is known to induce IL-8 release, resulted in a marked time-dependent exponential increase in neutrophil recruitment to the nasal chamber. When oxymetazoline (final concentration, 10(-5) M), was added to the superfusate, the PA induced-neutrophil migration was inhibited; the addition of benzalchoniul chloride to the superfusate had no effect on the PA induced-neutrophil migration. We conclude that a alpha2 adrenoceptor agonist profoundly inhibits neutrophil migration in response to bacterial products. Our novel method allows continuous introduction of stimuli and monitoring of responses in the nasal mucosa.

References

Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
May 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L GreiffC G Persson
Jan 20, 1994·Biochemical Pharmacology·I F Musgrave, R Seifert
Oct 1, 1994·American Journal of Respiratory and Critical Care Medicine·J A DouglassS T Holgate
Dec 1, 1994·American Journal of Respiratory Cell and Molecular Biology·H InoueJ A Nadel
Feb 1, 1994·The Journal of Allergy and Clinical Immunology·A TedeschiA Miadonna
Jan 1, 1996·American Journal of Respiratory and Critical Care Medicine·T KanekoJ A Nadel
Mar 1, 1996·The Annals of Otology, Rhinology, and Laryngology·M BendeM Intaglietta
Nov 1, 1995·Acta Oto-laryngologica·M AnderssonC Persson

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Citations

Aug 13, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ada María Herrera-GarcíaFederico Díaz-González

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