A Method for Cell Culture and Maintenance of Ammonia-Oxidizing Archaea in Agar Stab

Indian Journal of Microbiology
Yeon-Jin ChuGeun-Joong Kim


Ammonia oxidizing archaea (AOA) are predominantly found and closely linked with geochemical cycling of nitrogen in non-extreme habitats. However, these strains have mainly been investigated using liquid cultures of enriched cells. Here, we provide an agar stab as a simple and reliable means of cultivating and maintaining AOA.


Sep 24, 2005·Nature·Martin KönnekeDavid A Stahl
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Steven J HallamEdward F DeLong
Sep 28, 2010·Applied and Environmental Microbiology·Byoung-Joon ParkSung-Keun Rhee
Apr 14, 2011·Microbes and Environments·Naoki MatsutaniTatsuaki Tokuyama
Apr 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Maria TournaChrista Schleper
Nov 15, 2013·The ISME Journal·Man-Young JungSung-Keun Rhee

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