A method for construction of specialized transducing phage rho 11 of Bacillus subtilis

Gene
F KawamuraY Ikeda

Abstract

DNA from a temperate phage rho 11 and chromosomal DNA of Bacillus subtilis 168 were digested with endonuclease EcoRI and then ligated with T4 polynucleotide ligase. The ligated DNA fragments were used to transform a lysogenic strain, B. subtilis spoA12 lys21 hisA1 leuA8 p11, and Lys+, His+ or Leu+ transformants were selected. The cells of each type were then mixed, grown and treated with mitomycin C; the induced phages were tested for abilities abilities to form plaques and to tranduce the auxotrophic marker. Various types of plaque-forming or defective phages which transduce hisA or lys marker at considerably high frequencies were thus obtained.

References

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Citations

Jan 1, 1985·Molecular & General Genetics : MGG·M Fujita, Y Kobayashi
Jan 1, 1980·Molecular & General Genetics : MGG·F KawamuraY Ikeda
Aug 16, 1984·Biochemical and Biophysical Research Communications·J KudohK Kurahashi
Sep 1, 1991·Research in Microbiology·F Kunst, K Devine
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H ShimotsuH Saito
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May 4, 2017·Nature Communications·Marivic MartinÁkos T Kovács
Oct 1, 1986·Molecular & General Genetics : MGG·S YamashitaH Saito

Related Concepts

Natto Bacteria
Bacteriophages
DNA, Bacterial
Genes, Spliced
DNA, Viral
Histidine
Leucine
Enisyl
Transduction, Genetic
Transformation, Bacterial

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