A method for construction of specialized transducing phage rho 11 of Bacillus subtilis

doi:10.1016/0378-1119(79)90095-7

PMID: 109355DOI: 10.1016/0378-1119(79)90095-7Feb 1, 1979

A method for construction of specialized transducing phage rho 11 of Bacillus subtilis

Gene

F KawamuraY Ikeda

Abstract

DNA from a temperate phage rho 11 and chromosomal DNA of Bacillus subtilis 168 were digested with endonuclease EcoRI and then ligated with T4 polynucleotide ligase. The ligated DNA fragments were used to transform a lysogenic strain, B. subtilis spoA12 lys21 hisA1 leuA8 p11, and Lys+, His+ or Leu+ transformants were selected. The cells of each type were then mixed, grown and treated with mitomycin C; the induced phages were tested for abilities abilities to form plaques and to tranduce the auxotrophic marker. Various types of plaque-forming or defective phages which transduce hisA or lys marker at considerably high frequencies were thus obtained.

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TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE

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Related Concepts

Natto Bacteria

Bacteriophages

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A method for construction of specialized transducing phage rho 11 of Bacillus subtilis

Abstract

References

Organization of bacterial genes for ribosomal components: studies using novel approaches

Specialized transducing phages for ribosomal protein genes of Escherichia coli

Acquisition of thymidylate synthetase activity by a thymine-requiring mutant of Bacillus subtilis following infection by the temperate phage phi 3

Properties of the defective phage of Bacillus subtilis

Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage

TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE

Citations

Physical mapping of Bacillus subtilis phage rho14 cloning vehicles: heteroduplex and restriction enzyme analyses

Upper limit for DNA packaging by Bacillus subtilis bacteriophage phi 105: isolation of phage deletion mutants by induction of oversized prophages

Cloning of sporulation gene spoIVC in Bacillus subtilis

Isolation and mapping of a new suppressor mutation of an early sporulation gene spoOF mutation in Bacillus subtilis

Bacteriophage phi 1 as a gene-cloning vector in Bacillus subtilis

Identification of the sporulation gene spoOA product of Bacillus subtilis

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A physical map of the genome of the Bacillus subtilis temperate phage rho 11

Cloning vehicles for the homologous Bacillus subtilis host-vector system

A specialized transducing phage constructed from Bacillus subtilis phage phi 105

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Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis

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De novo evolved interference competition promotes the spread of biofilm defectors

The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions

Related Concepts

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