PMID: 9446705Apr 4, 1998Paper

A method for direct patch-clamp recording from smooth muscle cells embedded in functional brain microvessels

Pflügers Archiv : European journal of physiology
K Quinn, D J Beech

Abstract

The aim of this project was to develop a method to enable routine application of all patch-clamp configurations to smooth muscle cells while they remain embedded in blood vessels. Small blood vessels were isolated from rabbit brain using an enzymatic and mechanical procedure. Vessels were identified under a microscope and the majority were small arterioles with a mean external diameter, in Ca2+-containing (1.5 mM) solution, of 29 microm and variable lengths of 100 microm or more. Arterioles excluded trypan blue, constricted in response to 60 mM K+ and dilated in response to levcromakalim. Patch-clamp gigaOhm seals were made regularly on smooth muscle cells embedded in arterioles. The membrane potential recorded using amphotericin-B-containing patch pipettes averaged -72 mV. Short arteriolar segments could be voltage-clamped. Injection of depolarising current or bath application of 10 mM Ba2+ induced constriction of the entire arteriolar segment. Cell-attached patch, inside-out patch and outside-out patch recordings were made readily and K+ channel unitary currents were studied. The method is readily applied and has several advantages over previous methods for the study of ion channels in smooth muscle cells. Notably, avoidance ...Continue Reading

Citations

Feb 20, 2013·European Journal of Pharmacology·Xin-Zhi LiZhi-Gen Jiang
Nov 1, 2001·Journal of Pharmacological and Toxicological Methods·E E DanielL Janssen
Aug 27, 2005·Investigative Ophthalmology & Visual Science·Mary K McGahonTim M Curtis
Sep 21, 2013·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Ke-Tao MaJun-Qiang Si
Nov 3, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Ke-Tao MaZhi-Gen Jiang
Jul 25, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Shang-Zhong XuDavid J Beech
Oct 20, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·F M Faraci, C G Sobey
Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·A CheongD J Beech
Jul 19, 2000·American Journal of Physiology. Heart and Circulatory Physiology·K QuinnD J Beech
May 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Z ZhangT L Pallone

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