PMID: 7097754Jan 1, 1982

A method for estimating free Ca within human red blood cells, with an application to the study of their Ca-dependent K permeability

The Journal of Membrane Biology
T J Simons

Abstract

Murphy, Coll, Rich and Williamson (J. Biol. Chem. 255:6600--6608, 1980) described a null-point method for estimating intracellular free Ca in liver cells. They used digitonin to lyse the cells in solutions of varying Ca concentration. This method has been adapted for use with human red cells. The values found are about 0.4 micron or micrometer Ca in fresh cells, and from 0.4 to 0.7 micron or micrometer Ca in blood-bank cells, at pH 7.2 and 37 degrees C. These are likely to be overestimates, and the errors and limitations of the method are discussed. Red cells may be loaded with Ca by metabolic depletion in Ca-containing solutions. Such cells have an elevated K permeability, and the relationships between free Ca, total Ca and K permeability were investigated, using 86Rb as a tracer for K. 86Rb flux studies show that the affinity of the K channel for Ca is the same in cells as in resealed ghosts where intracellular Ca can be controlled with Ca buffers, but the rate of tracer equilibration is 3-6 times faster in ghosts than in cells.

References

Sep 4, 1979·Biochimica Et Biophysica Acta·J Garcia-SanchoB Herreros
Mar 1, 1976·The Journal of Physiology·T J Simons
Mar 1, 1976·The Journal of Physiology·T J Simons
Dec 1, 1968·The Journal of Physiology·D G Harrison, C Long
Aug 1, 1980·The Journal of Physiology·P W Flatman, V L Lew
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G FiskumA L Lehninger
May 7, 1980·Biochimica Et Biophysica Acta·T J BeelerA Martonosi
Jul 1, 1980·Biochimica Et Biophysica Acta·R Y Tsien, T J Rink

Citations

Jan 1, 1987·The Journal of Membrane Biology·B Engelmann, J Duhm
Mar 1, 1987·Pflügers Archiv : European journal of physiology·F V Sepúlveda, S M Smith
Apr 29, 1992·Biochimica Et Biophysica Acta·L WuF F Vincenzi
Nov 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·J S Huntley, A C Hall
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S Watanabe, M J Phillips
Jun 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·P A Mole, C R Paterson
Jul 9, 1984·FEBS Letters·T J Simons
Sep 24, 1982·Biochimica Et Biophysica Acta·O Scharff, B Foder
Aug 1, 1985·European Journal of Clinical Investigation·U Walter, S Müller
May 29, 1986·Biochemical and Biophysical Research Communications·C H FryT Powell

Related Concepts

Calcium
Cell Membrane Permeability
Erythrocyte Survival
Red Cell Ghost
Erythrocytes
Hydrogen-Ion Concentration
Magnesium
Potassium
Rubidium

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