A Method for Inoculation and Evaluation of Rice Sheath Blight Disease

Plant Disease
Dong-Soo ParkYinong Yang

Abstract

Sheath blight of rice, caused by Rhizoctonia solani, is one of the most important rice diseases worldwide; however, no rice cultivar has been found to be completely resistant to this fungus. To facilitate detailed analysis of sheath blight resistance at genetic, molecular, biochemical, and functional genomic levels, new methods were developed for effective and uniform infection and accurate evaluation of the disease. The efficiency of R. solani infection was tested on two resistant (Tetep and Jasmine 85) and two susceptible (Chucheongbyeo, Junambyeo) cultivars using three different inoculum types (agar block, liquid cultured mycelia ball, and mycelia suspension). By covering the inoculated sheaths with aluminum foil to maintain humidity, 100% infection rate was achieved in this study. Liquid cultured mycelia balls caused significantly longer lesions (5.4 cm) than other types of inoculum, including agar block (2.4 cm) and mycelia suspension (1.6 cm). An improved method for evaluating sheath blight disease was selected by comparing two methods for evaluating disease severity among three partially resistant cultivars and five susceptible cultivars inoculated with liquid cultured mycelia balls. In addition, a new formula was develo...Continue Reading

References

Oct 24, 2008·Phytopathology·Fabrício A RodriguesGaspar H Korndörfer
Jul 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Z LiW D Park
Jul 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Z LiW D Park
Jul 1, 2002·Plant Disease·G C EizengaJ N Rutger
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Citations

May 7, 2019·Plant Disease·Yi ZhuSoum Sanogo
Sep 16, 2010·Phytopathology·Nguyen Đac KhoaHans Jørgen Lyngs Jørgensen
Nov 1, 2008·Plant Disease·B Prasad, G C Eizenga
Mar 21, 2020·Frontiers in Plant Science·Vidisha BistSuchi Srivastava
Jan 29, 2021·Frontiers in Plant Science·Ying-Hong LinSheng-Chi Chu
Mar 31, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Surabhi JasrotiaManmohan Sharma
Jun 9, 2021·Bulletin of Environmental Contamination and Toxicology·Chuanfei BianBaotong Li
Aug 30, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Shimin ZuoXuebiao Pan

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