PMID: 6405796Jun 16, 1983Paper

A method for isolating human plasma lecithin:cholesterol acyltransferase without using anti-apolipoprotein D, and its characterization

Biochimica Et Biophysica Acta
V Mahadevan, L A Soloff

Abstract

A method for isolating human plasma lecithin:cholesterol acyltransferase (EC 2.3.1.43) purified more than 50 000-fold is described. The crude enzyme obtained by initial ammonium sulfate and citric acid treatment of 21 of human plasma is subjected to repeated DEAE-cellulose chromatography to yield a preparation purified more than 600-fold. Hydroxyapatite chromatography of concentrates from this fraction using 0.5 mM phosphate buffer, pH 6.8, yields enzyme preparations purified more than 50 000-fold. The enzyme isolated by this procedure was free of apolipoprotein D, as shown by the absence of an arc in immunodiffusion with anti-apolipoprotein D. The enzyme showed a single band by polyacrylamide gel electrophoresis in the presence and absence of SDS. Upon analytical isoelectrofocusing the enzyme separated into three iso forms with isoelectric points below that of egg albumin (pI 4.6). The enzyme was characterized by a high content of glutamic acid, leucine and glycine, and a lower content of tyrosine. The enzyme possessed both transferase and phospholipase A2 activities and both activities show absolute requirement for apolipoprotein A-I. The purified enzyme was injected into Balb/c mice and the antiserum reacted both with the pu...Continue Reading

References

Jan 1, 1978·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·E GustowL A Soloff
Jan 1, 1978·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·K G VarmaJ Frohlich
Feb 1, 1973·Biochemical Medicine·A G LackoL A Soloff
Apr 16, 1973·Biochimica Et Biophysica Acta·S Batzri, E D Korn
Jan 1, 1981·The Journal of Clinical Investigation·J J AlbersC H Chen
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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Citations

Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M YamamotoM Sugano
Jun 1, 1990·The British Journal of Psychiatry : the Journal of Mental Science·D V JamesR G Priest
Feb 1, 1988·The British Journal of Psychiatry : the Journal of Mental Science·R M Haller, R H Deluty
Oct 1, 1986·The British Journal of Psychiatry : the Journal of Mental Science·L SolyomC Solyom
Apr 28, 2001·The Journal of Biological Chemistry·N SettasatianK A Rye
Jun 27, 1995·Biochimica Et Biophysica Acta·H Q LiangP J Barter
Sep 1, 1987·Journal of Biochemical and Biophysical Methods·L Holmquist
Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

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