A method for lactate and pyruvate determination in filter-paper dried blood spots

Journal of Chromatography. a
Chih-Kuang ChuangShuan-Pei Lin

Abstract

Lactic acidemia is commonly associated with severe diseases in pediatric patients. Quantitation of blood lactate and pyruvate is important for the diagnosis and clinical management. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using dried blood spots (DBS) was developed and could be used for simultaneous quantification of blood lactate and pyruvate. The applicability of the developed method was tested and confirmed by the regression analysis between LC-MS/MS method and enzymatic assay. Lactate and pyruvate were extracted from DBS obtained from 580 full-term, 120 pre-term infants (gestations ranging from 24 to 36 weeks), and 65 patients with suspected lactic acidemia, with methanolic internal standard (IS) solutions of sodium L-lactate-(13)C(3) and pyruvate-(13)C(3). An API-2000 LC-MS/MS system with multiple reaction monitoring (MRM) mode was applied. The within-run and between-run precisions (CV%) were determined and the results were 1.9% and 3.9% for lactate (n=20) and 5.7% and 7.3% for pyruvate (n=20). The linearity of lactate (r=0.9986) and pyruvate (r=0.9973) based on the IS was excellent. The parameter r squared (r(2)) of linear regression between LC-MS/MS method and enzymatic assay was 0.9405 for lac...Continue Reading

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Nov 29, 2005·Clinical Biochemistry·Chih-Kuang ChuangShuan-Pei Lin
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Oct 17, 2007·Biomedical Chromatography : BMC·William McKinnonPhilip J Hilton

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Citations

Sep 25, 2014·Mass Spectrometry Reviews·Michel WagnerGérard Hopfgartner
Apr 25, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Margarida CoelhoBruno Manadas
Jul 20, 2018·The Journal of Sports Medicine and Physical Fitness·Lorena Franco-MartínezPedro Jiménez-Reyes
Mar 22, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eugene F DiekmanSander M Houten
Apr 20, 2021·Molecular Genetics and Metabolism Reports·Eugène F DiekmanSander M Houten

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