A method for preparing and staining histological sections containing titanium implants for light microscopy.

Stain Technology
K GotfredsenL N Jensen

Abstract

An improved method for preparing and staining ground tissue-implant sections for light microscopy is presented. Undecalcified tissue blocks with titanium implants were dehydrated in an ascending series of ethanol and stained in toto with basic fuchsin. Specimens were infiltrated and embedded in methyl methacrylate and sections were prepared using a cutting-grinding-system. The polished surface was counterstained with light green or anilin blue. Light polymerizing resin was used as slide mounting medium and for mounting the coverglass. The sections obtained were 10-15 microns thick with tissue architecture which clearly differentiated structures at the tissue-implant interface. The method was very useful for computer assisted morphometric analysis.

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Citations

Sep 1, 2005·International Orthopaedics·O RahbekK Søballe
Oct 27, 2009·International Orthopaedics·Thomas Bo JensenK Søballe
Jan 5, 2011·The Journal of Bone and Joint Surgery. British Volume·H DaugaardK Soballe
Feb 1, 1990·Acta Odontologica Scandinavica·E Budtz-Jörgensen
Apr 4, 2002·Acta Orthopaedica Scandinavica·Thomas B JensenKjeld Søballe
Nov 19, 2003·Acta Orthopaedica Scandinavica·Søren KoldKjeld Søballe
Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·L N JensenK Gotfredsen
Jul 1, 1992·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·R Crespi, S G Grossi
Jan 17, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Brian ElmengaardKjeld Søballe
Aug 30, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Thomas JakobsenKjeld Søballe
Aug 1, 2012·Journal of Biomedical Materials Research. Part a·Kasra ZainaliKjeld Soballe
Feb 4, 2010·Journal of Biomedical Materials Research. Part a·Stig S JakobsenKjeld Soballe
Jul 31, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jeppe BarckmanKjeld Soballe
Mar 8, 2006·The Journal of Arthroplasty·Søren KoldKjeld Søballe
Apr 30, 2013·Acta Orthopaedica·Jeppe BarckmanKjeld Søballe
Aug 18, 2005·Journal of Biomedical Materials Research. Part a·Brian ElmengaardKjeld Søballe
Feb 24, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Thomas B JensenKjeld Søballe
Apr 22, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T B JensenK Søballe
Oct 5, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Rasmus CleemannJorgen Baas
Jan 12, 2001·Journal of Biomedical Materials Research·O MouzinJ E Bechtold
Jul 19, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Søren KoldKjeld Søballe
Jul 19, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Søren KoldKjeld Søballe
Dec 21, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Thomas JakobsenKjeld Søballe
Dec 18, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M F Kunrath, R Hübler
Mar 9, 2005·Journal of Biomedical Materials Research. Part a·O RahbekK Soballe
Sep 11, 2012·The Open Orthopaedics Journal·Mikkel SaksøKjeld Søballe
Mar 9, 2006·Clinical Orthopaedics and Related Research·Thomas JakobsenKjeld Søballe

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