A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media

Frontiers in Bioengineering and Biotechnology
Clément BernyImène Chebbi

Abstract

We report the synthesis in large quantity of highly pure magnetosomes for medical applications. For that, magnetosomes are produced by MSR-1 Magnetospirillum gryphiswaldense magnetotactic bacteria using minimal growth media devoid of uncharacterized and toxic products prohibited by pharmaceutical regulation, i.e., yeast extract, heavy metals different from iron, and carcinogenic, mutagenic and reprotoxic agents. This method follows two steps, during which bacteria are first pre-amplified without producing magnetosomes and are then fed with an iron source to synthesize magnetosomes, yielding, after 50 h of growth, an equivalent OD565 of ~8 and 10 mg of magnetosomes in iron per liter of growth media. Compared with magnetosomes produced in non-minimal growth media, those particles have lower concentrations in metals other than iron. Very significant reduction or disappearance in magnetosome composition of zinc, manganese, barium, and aluminum are observed. This new synthesis method paves the way towards the production of magnetosomes for medical applications.

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Citations

Jun 25, 2020·International Journal of Molecular Sciences·Edouard Alphandéry
Oct 22, 2020·Microbial Cell Factories·Abdul BasitWei Jiang
Sep 6, 2020·Applied and Environmental Microbiology·Matthieu AmorArash Komeili
Nov 11, 2020·Microbial Cell Factories·Cornelius N RieseDirk Schüler
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Alexander Pekarsky, Oliver Spadiut
Aug 29, 2021·Pharmaceutics·Slavko Kralj, Silvia Marchesan
Jun 27, 2020·International Journal of Pharmaceutics·Edouard Alphandéry

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