A method for purification and viral inactivation of human placenta hemoglobin

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Tao LiChengmin Yang

Abstract

For pilot-scale manufacturing of hemoglobin-based oxygen carrying drugs, we should get highly pure and viral inactivated hemoglobin (Hb) at high recovery. In our method, placenta hemoglobin (PHb) solutions were purified by heating in the presence of reducing agent and deoxygenating conditions so that heat-sensitive proteins were selectively precipitated and virus was inactivated. The optimum preparative condition resulted in highly purified PHb solution (>99% pure) with approximate 90% recovery and less than 2% of MetHb content, maintained oxygen carrying capacity, residual phospholipids less than 1 ppm, free of endotoxin, bacteria, type A&B antigens and virus. Finally, we compared the efficacy of blood exchange on rat with poly-PHb and poly-Hb from adult blood. The results showed no significant difference between two products. Therefore, the placenta Hb obtained from this method could be supplied as materials for oxygen carrying drugs.

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Citations

Mar 27, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Yaojin LiChengmin Yang
Jul 2, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Shuang ZhangTao Li
Mar 15, 2012·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Tao LiJin Liu
Nov 17, 2012·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Tao LiJin Liu
Dec 4, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Lanzhen ZhaoChengmin Yang
Nov 10, 2011·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Tao LiChengmin Yang
Nov 9, 2006·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Thomas Ming Swi Chang
Sep 6, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Tao LiJin Liu
Jul 22, 2009·Biotechnology Progress·Jacob ElmerAndre F Palmer
Feb 19, 2009·Biotechnology Progress·Andre F PalmerDavid R Harris
Jul 4, 2006·Trends in Biotechnology·Thomas Ming Swi Chang
Jun 1, 2011·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Yuxin WangChengmin Yang
Mar 20, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Tao LiChengmin Yang
Jul 6, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Zhen YouYong Zeng
May 6, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Zhilan XieHongli Zhu
Jan 10, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Tao LiChengmin Yang
Nov 14, 2018·Current Medicinal Chemistry·Kazuaki TaguchiMasaki Otagiri
Oct 31, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Huiya MaKunping Yan
Jun 24, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Wei WuChengmin Yang
Sep 21, 2021·Cardiovascular Toxicology·Alireza JenabiMurugan Ramalingam

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