PMID: 8939795Jan 1, 1997Paper

A Method for Selection and Characterization of Rhizosphere-Competent Bacteria of Chickpea

Current Microbiology
C S Nautiyal

Abstract

A greenhouse assay was developed to evaluate the root-colonizing capability of the native chickpea rhizospheric bacterial population. In this assay system, screening time was reduced on two counts. First, spontaneous chromosomal rifampicin-resistant (Rifr) strains were directly inoculated to seeds without any check for the stability of the mutation, and second, no attempts were made to taxonomically identify all the strains being screened for chickpea rhizosphere competence. Only two chickpea rhizosphere-competent Rifr strains from the group of six good chickpea rhizosphere colonizers forming 10(7) to 10(8) colony-forming units (cfu)/g root were taxonomically identified as Pseudomonas fluorescens NB13R and Pseudomonas spp. NB49R, after screening 49 bacteria. Both the strains showed no difference from their corresponding wild-type strains P. fluorescens NB13 and Pseudomonas spp. NB49 in terms of chickpea rhizosphere competence. Isogenic or equally rhizospheric competitive second non-isogenic bacterial isolate, when present in tenfold higher amount, pre-empted the colonization of the soil by the bacterium, which was present in smaller ratio. These findings indicate that the isogenic or equally rhizospheric competitive second non-...Continue Reading

Citations

Jul 20, 2002·Trends in Biotechnology·Berndt Gerhardson
Jan 6, 2000·FEMS Microbiology Letters·C S NautiyalD Verma
Dec 27, 2002·The Journal of General and Applied Microbiology·Suneeta Kulkarni, Chandra Shekhar Nautiyal
Sep 18, 2008·Journal of Basic Microbiology·Neelam Tank, Meenu Saraf
Dec 17, 2009·Letters in Applied Microbiology·P S Chauhan, C S Nautiyal
Nov 18, 2016·Antonie van Leeuwenhoek·Shashank Kumar MishraPuneet Singh Chauhan
Mar 29, 2020·Frontiers in Microbiology·Richa SharmaPascal Piveteau

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