A method for studies on interactions between a gold-based drug and plasma proteins based on capillary electrophoresis with inductively coupled plasma mass spectrometry detection

Analytical and Bioanalytical Chemistry
Tam T T N NguyenBente Gammelgaard

Abstract

An analytical method based on capillary electrophoresis (CE) and inductively coupled plasma mass spectrometry (ICP-MS) detection was developed for studies on the interaction of gold-containing drugs and plasma proteins using auranofin as example. A detection limit of 18 ng/mL of auranofin corresponding to 5.2 ng/mL Au and a precision of 1.5 % were obtained. Kinetic studies of the interaction between auranofin and protein were performed by incubation in aqueous solutions as well as 20 % human plasma at 37 °C. The reaction of auranofin with human serum albumin (HSA) and plasma proceeded fast; 50 % of un-bound auranofin disappeared within 2 and 3 min, respectively. By blocking the free cysteine (Cys-34) by iodoacetamide on HSA, it was shown that Cys-34 was the main reaction site for auranofin. By selective labeling of HSA present in 20 % human plasma with iophenoxate, it was demonstrated that HSA was the major auranofin-interacting protein in plasma. The CE-ICP-MS method is proposed as a novel approach for kinetic studies of the interactions between gold-based drugs and plasma proteins. Graphical Abstract Development of a CE-ICP-MS based method allows for studies on interaction of the gold containing drug auranofin with plasma pro...Continue Reading

References

Oct 1, 1983·Annals of the Rheumatic Diseases·D LewisW E Smith
Dec 30, 1983·The American Journal of Medicine·K Blocka
Feb 1, 1984·Seminars in Arthritis and Rheumatism·R C BlodgettR G Pietrusko
Feb 27, 2007·Free Radical Biology & Medicine·Christine MarzanoMaria Pia Rigobello
Nov 8, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ivano Bertini, Gabriele Cavallaro
Mar 28, 2008·Bioinorganic Chemistry and Applications·Dick de VosEdward R T Tiekink
Aug 12, 2009·Biochemical Pharmacology·Valentina GandinCristina Marzano
Apr 7, 2012·Analytical and Bioanalytical Chemistry·Björn Meermann, Michael Sperling
Oct 12, 2012·Analytical and Bioanalytical Chemistry·Tam T T N NguyenBente Gammelgaard
Apr 16, 2013·International Journal of Pharmaceutics·Tam T T N NguyenBente Gammelgaard
Feb 5, 2015·Analytical and Bioanalytical Chemistry·Julie Maria DerschBente Gammelgaard

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Citations

Feb 28, 2018·Metallomics : Integrated Biometal Science·Hannah U HoltkampChristian G Hartinger
Oct 27, 2016·Electrophoresis·Anna TýčováKarel Klepárník
Aug 16, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Giarita FerraroDaniela Marasco

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