A method for studying oxygen diffusion barrier in erythrocytes: effects of haemoglobin content and membrane cholesterol

The Journal of Physiology
K KonT Suda

Abstract

1. In order to study the kinetics os the oxygen egress from human red cells in the 50 sec-20 min time range, an apparatus for measuring the oxygen dissociation process was constructed, combining a spectrophotometer with an oxygen electrode of quick response. 2. Starting from air-saturated haemolysate or red cell suspensions, the velocity of oxygen dissociation from oxyhaemoglobin (Vdiss) and of oxygen disappearance in the medium (Vobs) after addition of bakers' yeast (consuming the dissolved oxygen at the velocity of Vconsump) were recorded. A parameter (r) was defined as the ratio of two velocities, Vegress (the velocity of oxygen egress into the medium) and Vdiss, r identical to Vegress/Vdiss = (Vconsump -Vobs)/Vdiss. Vcomsump could be calculated by the Michaelis-Menten equation as follows, Vconsump = Vmax [O2]/(Km + [O2]), where Vmax was the maximal velocity of oxygen consumption of bakers' yeast. 3. The r value was always 1.0 for the haemolysate, but it was less than 1.0 for the normal red cells. Further, the oxygen dissociation curve of red cells obtained at higher Vmax was distorted, due to the non-equilibration between intra- and extracellular oxygen concentrations. 4. The r value was (i) independent of the amounts of th...Continue Reading

Citations

Dec 16, 2000·Clinical and Experimental Pharmacology & Physiology·H BuchwaldT D Rohde
Oct 22, 1998·Surgery·H J MenchacaH Buchwald
Nov 21, 2000·Journal of the American College of Surgeons·H BuchwaldT J O'Dea
May 1, 2007·Environmental Toxicology and Pharmacology·Karine PortierJean Coudert
Oct 1, 1982·Pflügers Archiv : European journal of physiology·K KonT Shiga
Aug 1, 1987·Environmental Health Perspectives·N MaedaT Shiga
Nov 19, 2019·Frontiers in Physiology·Juan Pablo Zuniga-Hertz, Hemal H Patel
Jan 1, 1990·Critical Reviews in Oncology/hematology·T ShigaK Kon

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