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A Method for Targeted 16S Sequencing of Human Milk Samples

Journal of Visualized Experiments : JoVE

Mar 23, 2018

Nicole H. TobinGrace M. Aldrovandi

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Abstract

Studies of microbial communities have become widespread with the development of relatively inexpensive, rapid, and high throughput sequencing. However, as with all these technologies, reproducible results depend on a laboratory workflow that incorporates appropriate precautions and cont...read more

Mentioned in this Paper

Polymerase Chain Reaction
RNA, Ribosomal, 16S
Study
Extraction
DNA Amplification
Cow milk allergenic extract
DNA, Bacterial
Nucleic Acids
Sequence Determinations, DNA
Bacterial 16S RNA
3
5
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A Method for Targeted 16S Sequencing of Human Milk Samples

Journal of Visualized Experiments : JoVE

Mar 23, 2018

Nicole H. TobinGrace M. Aldrovandi

PMID: 29630048

DOI: 10.3791/56974

Abstract

Studies of microbial communities have become widespread with the development of relatively inexpensive, rapid, and high throughput sequencing. However, as with all these technologies, reproducible results depend on a laboratory workflow that incorporates appropriate precautions and cont...read more

Mentioned in this Paper

Polymerase Chain Reaction
RNA, Ribosomal, 16S
Study
Extraction
DNA Amplification
3
5

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