PMID: 6536820Sep 1, 1984Paper

A method for the assay of alpha 1-acid glycoprotein in dog serum and its application to the plasma binding of propranolol and oxprenolol in animals receiving rifampicin

Journal of Pharmacological Methods
C DelcroixF M Belpaire

Abstract

A method for determination of alpha 1-acid glycoprotein (alpha 1-AGP) in dog serum was developed. Dog alpha 1-AGP was purified to homogeneity from pooled serum by DEAE and CM ion exchange chromatography. A monospecific antibody was obtained by injecting the antigen in rabbits. The antibody was used in a turbidimetric method that allowed detection at 10 micrograms/ml in serum. A linear correlation was found between the optical density and alpha 1-AGP concentration up to 20 micrograms alpha 1-AGP in the sample. The method has been used to demonstrate the effect of treatment with rifampicin on the alpha 1-AGP serum concentrations and its relation to the protein binding of oxprenolol and propranolol.

References

May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Jan 1, 1982·European Journal of Clinical Pharmacology·F M BelpaireM Rosseneu
May 1, 1980·Clinical Pharmacokinetics·K M Piafsky
Aug 1, 1983·British Journal of Clinical Pharmacology·J FeelyE Guy
Nov 1, 1983·British Journal of Clinical Pharmacology·R J HermanA J Wood
May 1, 1982·Biochemical Pharmacology·M Brinkschulte, U Breyer-Pfaff
Nov 1, 1981·British Journal of Clinical Pharmacology·P A RoutledgeD G Shand

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Citations

Jul 17, 2008·Veterinary Research Communications·M YukiK Takase
Dec 1, 1987·Journal of Pharmacological Methods·C P DelloN H Fraeyman
Mar 1, 1987·Journal of Veterinary Pharmacology and Therapeutics·F M BelpaireM G Bogaert

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