A method for the extraction of ammonium from freshwaters for nitrogen isotope analysis

Analytical Chemistry
M F LehmannJ A McKenzie

Abstract

The measurement of delta15N values of inorganic nitrogen species is an important analytical tool to trace nitrogen species in order to understand nitrogen cycling in aquatic systems. Nitrogen isotope analysis of freshwater ammonium has, however, been hindered by the lack of a simple and reliable technique to measure delta15N values at natural abundance levels. We present a simple and rapid method to concentrate ammonium from freshwater samples for on-line N-isotope ratio determination. Ammonium is collected by adsorption on N-free cation exchange resins. The dried N-loaded exchange resin is then directly combusted to produce N2 gas for subsequent delta15N analysis. The method was evaluated with simulated freshwater solutions containing varying amounts of standard NH4+-N (delta15N = 2.1 per thousand) and potentially interfering inorganic and organic compounds. In general, the cation exchange resin method gives accurate and reproducible delta15N values (sigma1 < 0.3 per thousand; n = 10). Because of adsorption interference, high concentrations of cations in solution may cause ammonium loss but do not result in measurable isotope fractionation. Replicate extractions of the ammonium standard added to water collected from four Swiss...Continue Reading

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Citations

Mar 25, 2014·Analytical Chemistry·Dongwei LiuYuepeng Pan
Jul 16, 2014·Water Research·Jani M SalminenTaina H Nystén
Nov 17, 2010·Rapid Communications in Mass Spectrometry : RCM·Petra LachouaniWolfgang Wanek
Jul 12, 2019·Rapid Communications in Mass Spectrometry : RCM·Patricia StockDiana Burghardt
Oct 24, 2007·Analytical Chemistry·Dries HuygensOswald Van Cleemput
Jul 16, 2004·Journal of Environmental Quality·Michal Segal-RozenhaimerAvi Shaviv
Jun 6, 2006·Environmental Science & Technology·Miriam ReinhardtBernhard Wehrli
Sep 30, 2021·Analytical Methods : Advancing Methods and Applications·Wei GuoJun Li

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