PMID: 8957204Nov 1, 1996Paper

A method for the isolation and preparation of surfactant from tracheobronchial aspirates in infants and children for quantitative and pulsating bubble analysis

European Journal of Clinical Investigation
K RaymondosH von der Hardt

Abstract

A method is presented for the investigation of surfactant function after isolation and preparation of pulmonary surfactant from tracheobronchial aspirates of mechanically ventilated infants and children. This method involved purification of surfactant from contaminating non-surfactant phospholipids and mucus by sodium bromide density gradient centrifugation. The surfactant concentration in undiluted fluids (3.5 +/- 0.8 mg mL-1; mean +/- SD) was calculated by extrapolating from the urea concentration in aspirates and serum. Phospholipid concentration was adjusted to approximately 3 mg mL-1 and the surface activity was determined by means of a pulsating bubble surfactometer. Mean minimal and maximal tension values of all cycles were calculated after reaching steady state. Measurements obtained from surfactant isolated with this method showed a high reproducibility: the coefficient of variation for minimal surface tension was 6.1%. Therefore this method enables functional surfactant analysis in tracheobronchial aspirates.

Citations

Mar 16, 2004·Lancet·Caroline G P Roberts, Paul W Ladenson
Jul 24, 1998·Burns : Journal of the International Society for Burn Injuries·N PalluaA Berger
Oct 4, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Wolfgang Bernhard

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