A method for the isolation and serial propagation of keratinocytes, endothelial cells, and fibroblasts from a single punch biopsy of human skin

In Vitro Cellular & Developmental Biology. Animal
J Normand, M A Karasek

Abstract

When multiple types of cells from normal and diseased human skin are required, techniques to isolate cells from small skin biopsies would facilitate experimental studies. The purpose of this investigation was to develop a method for the isolation and propagation of three major cell types (keratinocytes, microvascular endothelial cells, and fibroblasts) from a 4-mm punch biopsy of human skin. To isolate and propagate keratinocytes from a punch biopsy, the epidermis was separated from the dermis by treatment with dispase. Keratinocytes were dissociated from the epidermis by trypsin and plated on a collagen-coated tissue culture petri dish. A combination of two commercial media (Serum-Free Medium and Medium 154) provided optimal growth conditions. To isolate and propagate microvascular endothelial cells from the dermis, cells were released following dispase incubation and plated on a gelatin-coated tissue culture dish. Supplementation of a standard growth medium with a medium conditioned by mouse 3T3 cells was required for the establishment and growth of these cells. Epithelioid endothelial cells were separated from spindle-shaped endothelial cells and from dendritic cells by selective attachment to Ulex europeus agglutinin I-coat...Continue Reading

References

Aug 1, 1978·The Journal of Investigative Dermatology·S C Liu, M Karasek
Jun 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E W JohnsonN L Parenteau
Mar 1, 1991·Differentiation; Research in Biological Diversity·B H LiptonM A Karasek
Feb 1, 1990·Journal of Cellular Physiology·R M TuderK G Bensch
Jan 1, 1987·Differentiation; Research in Biological Diversity·S KumarA Ager
Jun 1, 1989·The Australian and New Zealand Journal of Surgery·A F BreidahlG J Clunie
Sep 1, 1986·Clinical Endocrinology·H E Kulin, S J Santner
Nov 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R M MarksR Penny
Jan 1, 1983·Journal of Ultrastructure Research·K G BenschM A Karasek
Jun 1, 1983·Cell Biology International Reports·M HormiaI Virtanen
Aug 1, 1980·The Journal of Investigative Dermatology·P Hawley-NelsonS H Yuspa
Oct 1, 1980·The Journal of Investigative Dermatology·P M DavisonM A Karasek
Feb 1, 1982·Journal of Cellular Physiology·M C TsaoR G Ham
Jul 1, 1980·Experimental Cell Research·B R McAuslanW Reilly
Apr 1, 1993·In Vitro Cellular & Developmental Biology. Animal·P W HewettM Woodcock
Nov 1, 1993·In Vitro Cellular & Developmental Biology. Animal·P W Hewett, J C Murray

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Citations

Oct 27, 1997·Journal of Cellular Physiology·L I RomeroM A Karasek
Aug 2, 2012·Cell and Tissue Banking·J DragúňováJ Koller
Nov 26, 2010·In Vitro Cellular & Developmental Biology. Animal·Mahipal Singh, Anil K Sharma
Jun 5, 2003·Experimental and Molecular Pathology·Yukari HattoriJames Varani
Feb 20, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Nanjun WuJeffrey M Davidson
Feb 4, 2011·The New England Journal of Medicine·Cynthia St HilaireManfred Boehm
Sep 1, 1996·The Journal of Experimental Medicine·M VoraM A Karasek
Jan 19, 2013·American Journal of Respiratory and Critical Care Medicine·Maylis DagouassatJorge Boczkowski
Sep 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Howard Y ChangPatrick O Brown
Jun 5, 2001·The British Journal of Radiology·G J van den AardwegW J Mooi
May 26, 2012·Cellular and Molecular Life Sciences : CMLS·Cristina SanduMarie-Paule Felder-Schmittbuhl
Dec 3, 2014·The American Journal of Pathology·Susan E SamarasJeffrey M Davidson
Jan 15, 2013·The Journal of Investigative Dermatology·Ananya Datta MitraSmriti Kundu-Raychaudhuri
Mar 14, 2008·The Journal of Investigative Dermatology·John L RinnHoward Y Chang
Mar 30, 2007·The Journal of Investigative Dermatology·Karin M AbergTheodora M Mauro
Dec 24, 2005·The Journal of Investigative Dermatology·Sirosh M BokhariVaishali Chaudhuri
Dec 24, 2005·The Journal of Investigative Dermatology·Jie LiM Peter Marinkovich
Jul 24, 2007·Molecular Genetics and Metabolism·Jin-Song ShenChristine R Kaneski
Dec 10, 2014·Scientific Reports·Venkat Raghavan Krishnaswamy, Purna Sai Korrapati
Dec 2, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Lara K ParkJonathan A Garlick
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jin Yu LiuGünter Burg
Apr 17, 2013·Biotechnology and Applied Biochemistry·Sareh PandamoozAmrollah Mostafazadeh
Jul 19, 2013·Journal of Cellular and Molecular Medicine·Yongqing Wang, Bashar Kahaleh
Jan 20, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ling TangJie Li
Mar 20, 2008·The American Journal of Pathology·Siba P RaychaudhuriSmriti K Raychaudhuri
May 10, 2007·Journal of Biomedicine & Biotechnology·Frank JacobsenLars Steinstraesser
Jun 25, 2015·Nature Communications·Maja SedicCharlotte Kuperwasser
Apr 23, 2013·Biomatter·Michael J Susienka, Damian Medici
Jul 31, 2012·Cytokine·Anupam MitraSiba P Raychaudhuri
Feb 7, 2015·Journal of Materials Science. Materials in Medicine·K KarthikeyanPurna Sai Korrapati
Jun 23, 2016·Scientific Reports·Isabel OnofreLuis Pereira de Almeida

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