PMID: 8961565Dec 1, 1996Paper

A method for the large scale isolation of high transformation efficiency fungal genomic DNA

FEMS Microbiology Letters
D ZhangC E Cerniglia

Abstract

A procedure for isolation of genomic DNA from the zygomycete Cunninghamella elegans and other filamentous fungi and yeasts is reported. This procedure involves disruption of cells by grinding using dry ice, removal of polysaccharides using cetyltrimethylammonium bromide and by phenol extractions, and precipitation of DNA with isopropanol at room temperature. The isolation method produced large scale (approximate 1 mg DNA/5 g wet cells) and highly purified high molecular mass DNA. Sau3AI partially digested DNA showed high transformation efficiency (> 10(6)/100 ng DNA) when ligated to ZAP-express lambda vector.

References

Sep 1, 1991·Medicinal Research Reviews·A M Clark, C D Hufford
Nov 1, 1994·Applied and Environmental Microbiology·C E CernigliaR H Dodge

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Citations

Sep 10, 2013·Fungal Biology·Ran ChaiLiyou Qiu
Oct 25, 2000·Journal of Microbiological Methods·J TaubertR G Berger
Mar 26, 2014·Veterinary Parasitology·Wendell M S PerinottoVânia R E P Bittencourt
Aug 14, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Vanessa da S RamosMaria L R Macedo
Sep 2, 2008·The Journal of Microbiology·Chun Yan WangChang-Keun Sung
Jan 23, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fuqian WangYonghui Zhang
Dec 21, 2014·Current Microbiology·Jalal Soltani, Mahdieh S Hosseyni Moghaddam
Jun 2, 2009·Journal of Industrial Microbiology & Biotechnology·Kaihui LiuWenqiang Chen
Dec 29, 2010·Current Microbiology·Sonia A BottonJanio Morais Santurio
Nov 26, 2010·Journal of Industrial Microbiology & Biotechnology·Ya WangDu Zhu
Nov 27, 2010·Applied Microbiology and Biotechnology·Jinkui YangKe-Qin Zhang
Apr 13, 2013·World Journal of Microbiology & Biotechnology·Xiaowei DingFeihu Liu
Sep 13, 2014·World Journal of Microbiology & Biotechnology·Shaohua ShuMo Wang
Jan 28, 2014·Environmental Science and Pollution Research International·Diansi YuChanghong Guo
Feb 12, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lingli CuiShaohua Shu
Oct 30, 2016·Journal of Microbiological Methods·Mebeaselassie AndargieJianxiong Li

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