PMID: 3144Feb 1, 1976

A method for the regulation of microbial population density during continuous culture at high growth rates

Archives of Microbiology
G A Martin, W P Hempfling

Abstract

A method for the continuous culture of microorganisms is described which employs growth-dependent pH changes to control the rate of addition of fresh medium to a culture vessel. The apparatus (the "phauxostat") supports, at constant pH, long-term continuous culture at rates near or at the maximum of which the organisms are capable. The buffering capacity of the inflowing medium determines the steady-state population density of the culture, but the rate of growth is independent of the buffering capacity. The fundamental theory of operation is tested and some basic parameters of growth are estimated using Escherichia coli B growing continuously in media containing glucose, glycerol or DL-lactate.

References

Oct 9, 1970·Biochemical and Biophysical Research Communications·W P Hempfling
Jul 1, 1953·The Journal of General Physiology·P A ANDERSON
Sep 20, 1954·The Journal of General Physiology·J H NORTHROP
Jul 1, 1956·Journal of General Microbiology·D HERBERTR C TELLING
Jul 18, 1952·Science·V Bryson, W Szybalski

Citations

Jul 1, 1990·Biotechnology and Bioengineering·G LarssonH Pham
Dec 1, 1976·Archives of Microbiology·A H Stouthamer, C W Bettenhaussen
Nov 19, 2010·Journal of Industrial Microbiology & Biotechnology·Pavel Kyslík, Aleš Prokop
Feb 24, 2010·Biomedical Microdevices·Xianjia LuoYong Chen
Sep 1, 1991·Journal of Biotechnology·P LicariR Swartz
Dec 18, 2008·The FEBS Journal·Philip GroeneveldHans V Westerhoff
Jul 30, 2015·Microbiology·Kaarel AdambergRaivo Vilu
Jul 22, 2015·PloS One·Dominick MatteauSébastien Rodrigue
Dec 26, 2001·Biotechnology and Bioengineering·Peter G Verschuren, Johannes C van den Heuvel
Apr 5, 1989·Biotechnology and Bioengineering·I G MinkevichV K Eroshin
Oct 9, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Yang WeiRobert A Latour
Jun 1, 1978·Biotechnology and Bioengineering·L F OltmannA H Stouthamer
Jul 23, 1999·Biotechnology and Bioengineering·S A Siano
Feb 1, 1985·Biotechnology and Bioengineering·C W Rice, W P Hempfling
Mar 24, 2018·BioMed Research International·Svetlana V KamzolovaIgor G Morgunov

Related Concepts

Bacteriological Techniques
Ampholytes
Alkalescens-Dispar Group
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