A method for the simultaneous determination of mercapturic acids as biomarkers of exposure to 2-chloroprene and epichlorohydrin in human urine

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Elisabeth EckertThomas Göen

Abstract

We developed and validated an analytical method for the simultaneous determination of several chlorine and non-chlorine containing mercapturic acids in urine as specific metabolites of the hazardous chemicals 2-chloroprene and epichlorohydrin. The method involves an online column switching arrangement for online solid phase extraction of the analytes with subsequent analytical separation and detection using LC-MS/MS. The developed method enables for the first time the determination of Cl-MA-I (4-chloro-3-oxobutyl mercapturic acid), Cl-MA-II (4-chloro-3-hydroxybutyl mercapturic acid), Cl-MA-III (3-chloro-2-hydroxy-3-butenyl mercapturic acid) and HOBMA (4-hydroxy-3-oxobutyl mercapturic acid) as potential biomarkers of 2-chloroprene in urine. Additionally, CHPMA (3-chloro-2-hydroxypropyl mercapturic acid) as a specific metabolite of epichlorohydrin in urine and DHBMA (3,4-dihydroxybutyl mercapturic acid) can be determined. The analytical method proved to be both sensitive and reliable with detection limits ranging from 1.4 μg/L (for Cl-MA-III) to 4.2 μg/L (for HOBMA). Intra- and interday imprecision was determined to range from 4.7 to 11.8%. Due to the good accuracy and precision and the low limits of detection the developed metho...Continue Reading

References

Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ralf PreussJürgen Angerer
Feb 9, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·T SchettgenT Kraus
Sep 22, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elisabeth EckertThomas Göen
Feb 23, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C MoschW Völkel
Nov 16, 2010·Journal of Environmental Monitoring : JEM·Helle R HansenAndrew A Meharg

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Citations

Feb 6, 2013·Archives of Toxicology·Elisabeth EckertThomas Göen
May 4, 2015·Analytical and Bioanalytical Chemistry·Nikola PluymMax Scherer
Sep 17, 2019·Risk Analysis : an Official Publication of the Society for Risk Analysis·Sonja N SaxKenneth A Mundt

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