PMID: 7025962Sep 28, 1981Paper

A method for the simultaneous estimation of regional rates of glucose influx and phosphorylation in rat brain using radiolabeled 2-deoxyglucose

Brain Research
V J Cunningham, J E Cremer

Abstract

A procedure is described which allows the simultaneous estimation of the rate of glucose influx from blood to brain and of the rate of glucose phosphorylation in discrete brain regions of the rat. The method is applicable over an experimental period of 5 min and involves sequential i.v. injections of [14C]2-deoxyglucose and [3H]2-deoxyglucose. Arterial blood samples are collected over the experimental period. After 5 min the rat is killed by microwave irradiation to the head and the brain dissected. The data obtained on glucose, 2-deoxyglucose and 2-deoxyglucosephosphate concentrations in plasma and brain are analyzed in terms of a model which relates the kinetics of 2-deoxyglucose uptake and phosphorylation to those of glucose. The model is based on measurements made of the relative rates of unidirectional influx of glucose and 2-deoxyglucose and of their relative fractional rates of phosphorylation.

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Citations

Mar 1, 1986·Metabolic Brain Disease·A L Miller
Jan 1, 1989·Bulletin of Mathematical Biology·G O Sperber
May 1, 1984·Brain Research·A L MillerD M Staton
Nov 19, 1993·Brain Research. Developmental Brain Research·C RogerA Nehlig
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May 23, 2012·ASN Neuro·Gerald A Dienel
Apr 1, 1985·Medical Informatics = Médecine Et Informatique·V J Cunningham
Jun 1, 1985·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A GjeddeG Pawlik
Jan 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H KuwabaraA Gjedde
Nov 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·F SchuierL Sokoloff
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Nov 11, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D A PelligrinoS I Harik
Jun 1, 1986·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·V J CunninghamS R Moorhouse
Apr 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J E CremerV J Cunningham
Sep 1, 1985·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·V J Cunningham
Mar 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H KuwabaraA Gjedde
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Jun 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K MoriL Sokoloff
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Jul 19, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Heiko BackesGünter Mies

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