PMID: 6989791Jan 1, 1980Paper

A method for the ultrastructural demonstration of non-specific esterase in human blood and lymphoid tissue

The Histochemical Journal
B C PayneH Rappaport

Abstract

Using the substrate 2-naphthylthiol acetate (NTA), we developed a reproducible method of demonstrating a non-specific esterase while retaining nuclear and cytoplasmic details at the ultrastructural level. The NTA esterase had a distribution and pattern of staining similar to those of esterases demonstrable at the light microscopic level by the alpha-naphthyl acetate or naphthol AS-D acetate esterase reaction. The NTA esterase appeared as intensely electron-dense granules of varying size and shape in the cytoplasm. The granules were most abundant in the cells of the histiomonocytic series. The large number of diffusely scattered granules in the cytoplasm of the histiocytes and monocytes made it possible to separate these cells from other haematopoietic elements. There was usually no direct relationship between the NTA esterase positivity and the amount or the location of lysosomes or mitochondria, although in some histiocytes the granules appeared to be associated with lysosomes. The NTA esterase-positive granules were usually more numerous than lysosomes and were located outside the lysosomal granules. Some of the lymphocytes outside the germinal centres and most of the lymphocytes in the blood showed a punctate positivity in t...Continue Reading

References

Jan 1, 1976·Acta Haematologica·J Corberand
May 1, 1977·Scandinavian Journal of Haematology·K E HiggyF G Hayhoe
Mar 1, 1978·American Journal of Clinical Pathology·G A PangalisH Rappaport
Jan 1, 1971·Acta Haematologica·F Schmalzl, H Braunsteiner
Jan 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C Y LiL T Yam
Jun 1, 1968·British Journal of Haematology·L RozenszajnJ Epstein
Mar 1, 1971·American Journal of Clinical Pathology·L T YamW H Crosby
Apr 17, 1969·Histochemie. Histochemistry. Histochimie·D C LivingstonP B Gahan
Nov 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bell, R J Barrnett
Sep 25, 1956·The Journal of Biophysical and Biochemical Cytology·H BEAUFAYE UNDERHAY
Dec 1, 1959·The Journal of Biophysical and Biochemical Cytology·G M LEHRER, L ORNSTEIN

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Citations

May 1, 1989·The Histochemical Journal·L H RademakersP I Compier-Spies
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·B AkikusaY Kondo
Jan 1, 1984·The Journal of Pathology·J Crocker, M Williams

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