A method for toxicity screening of biomaterials using cells cultured on millipore filters

Journal of Biomedical Materials Research
A WennbergL Tronstad

Abstract

In the present paper a tissue culture technique is described whereby the toxicity of setting and solid materials may be evaluated. A cell monolayer was established on a millipore filter which was placed on an agar medium, cell side down. Test specimens were placed on top of the millipore filter and were allowed to influence the cells through the filter for two hours. The cell reaction was assessed by incubating the cells, still adherent to the filter, for the demonstration of succinate dehydrogenase activity. Materials with a cytotoxic effect caused a zone of inhibited enzyme activity in the cell-material contact area. The filters were examined macroscopically and scores from 0 to 3 were given to grade the severity of the cell response. Unset and set silicate cement, zinc phosphate cement and an acrylic resin were tested. The results obtained were consistent and in accordance with those of previous reports. The method was simple and rapid and appeared suitable for the assay of larger test series.

References

Jan 1, 1978·Acta Odontologica Scandinavica·A WennbergL Tronstad
Oct 1, 1978·Journal of Endodontics·L TronstadG Hasselgren
Mar 1, 1976·Journal of Biomedical Materials Research·T N Salthouse
Jan 1, 1976·Acta Odontologica Scandinavica·A Wennberg
Mar 1, 1973·Oral Surgery, Oral Medicine, and Oral Pathology·L Spangberg
Nov 1, 1973·Oral Surgery, Oral Medicine, and Oral Pathology·L SpangbergK Langeland
Nov 1, 1973·Journal of Dental Research·M Nakamura, J C Thonard
Sep 1, 1970·Journal of Biomedical Materials Research·C A Homsy
Oct 1, 1965·Journal of Pharmaceutical Sciences·W L GuessJ Autian
Nov 1, 1956·The American Journal of Medicine·H EAGLE, G E FOLEY
Jan 1, 1965·Journal of Pharmaceutical Sciences·S A ROSENBLUTHJ AUTIAN
Aug 1, 1952·Proceedings of the National Academy of Sciences of the United States of America·R Dulbecco
Jan 1, 1968·The Journal of Cell Biology·R M McCombsJ P Brunschwig

❮ Previous
Next ❯

Citations

Jan 1, 1991·Dental Materials : Official Publication of the Academy of Dental Materials·M KagaT Okabe
Apr 1, 1982·Biomaterials·R Sharma, P Vasudevan
Jan 1, 1992·Biomaterials·T HanawaM Ota
May 1, 1996·Dental Materials : Official Publication of the Academy of Dental Materials·C T HanksZ Sun
Jan 23, 2003·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·David C ThomShimon Friedman
Dec 28, 1999·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·A C Ventura, M Böhnke
Jul 1, 1992·International Endodontic Journal·J R McNamaraO W Wiebkin
Nov 1, 1990·Journal of Oral Rehabilitation·I A Mjör
Jun 1, 1996·European Journal of Oral Sciences·H Schweikl, G Schmalz
Oct 1, 1994·Endodontics & Dental Traumatology·I D OwadallyR F Wilson
Jun 1, 1994·Endodontics & Dental Traumatology·B S ChongR F Wilson
Aug 1, 1996·Endodontics & Dental Traumatology·M Torabinejad, T R Pitt Ford
Jan 1, 1996·Journal of Biomaterials Science. Polymer Edition·Y KuroyanagiN Shioya
Jan 1, 1990·Biomaterials, Artificial Cells, and Artificial Organs·A WieslanderP Kjellstrand
Jan 1, 1983·Journal of Biomedical Materials Research·A WennbergA Hensten-Pettersen
Sep 1, 1985·Journal of Endodontics·C Yesilsoy, R J Feigal
Dec 9, 1993·Clinical Materials·G L Polyzois
Oct 1, 1992·Journal of Dentistry·A Watts, R C Paterson
Apr 1, 1988·Journal of Dentistry·A K JowettE C Combe
Oct 1, 1989·Endodontics & Dental Traumatology·D Arenholt-Bindslev, P Hörsted-Bindslev
Feb 1, 1985·Scandinavian Journal of Dental Research·P MilledingG Hasselgren
Sep 1, 1980·International Endodontic Journal·L Tronstad, A Wennberg
May 1, 1981·International Endodontic Journal·A Wennberg, G Hasselgren
Jan 1, 1981·International Endodontic Journal·J Leirskar, K Helgeland
Oct 1, 1982·International Endodontic Journal·S D MeryonR M Browne
Jul 1, 1984·International Endodontic Journal·G S Heithersay
Sep 1, 1988·Journal of Oral Pathology·C T HanksD H Pashley
Jun 25, 2014·TheScientificWorldJournal·Claudio PoggioLivia Visai
Nov 1, 1991·Journal of Dental Research·C T HanksR G Craig
Sep 1, 1988·Journal of Dental Research·M KagaT Okabe
Jul 1, 1993·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·T TsuchiyaA Nakamura
Nov 1, 1981·Journal of Biomedical Materials Research·J B Ulreich, M Chvapil
Mar 1, 1988·International Endodontic Journal·W M Murphy
Mar 1, 2002·Journal of Biomedical Materials Research·Joel D BumgardnerGabriele Leyhausen
Mar 1, 1988·International Endodontic Journal·A Hensten-Pettersen
Dec 1, 1995·Acta Odontologica Scandinavica·H LygreN R Gjerdet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.