PMID: 6164442Apr 6, 1981Paper

A method for tracing biochemically defined pathways in the central nervous system using combined fluorescence retrograde transport and immunohistochemical techniques

Brain Research
P E Sawchenko, L W Swanson

Abstract

A simple method for the simultaneous localization of an antigen and a retrogradely transported fluorescent dye within single neurons is described. The method is based upon: (1) the efficiency of retrograde neuronal labeling with the fluorescent marker 'true blue'; (2) the near-quantitative persistence of retrogradely transported true blue localizations after subsequent processing of the tissue for immunohistochemistry; and (3) the ability to distinguish clearly between true blue- and immunohistochemically-stained cells by simply using appropriate excitation wavelengths for each. First we examined the characteristics of two fluorescent tracers which are effectively transported over long distances in the rat. The results confirm that true blue and bisbenzimide are transported from terminal fields to parent cell bodies and that both tracers are taken up and transported by damaged fibers and by undamaged fibers-of-passage. No evidence for transneuronal transport of either dye in the anterograde or in the retrograde direction was found. Next, using the projection of the paraventricular nucleus of the hypothalamus (PVH) to the spinal cord as a test system, it was found by direct cell counts that a considerably greater percentage of c...Continue Reading

References

Feb 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M Mesulam
Nov 26, 1976·Brain Research·C B SaperW M Cowan
Apr 1, 1979·Neuroscience Letters·H G KuypersC E Catsman-Berrevoets
Jan 1, 1978·Annual Review of Neuroscience·E G Jones, B K Hartman
Nov 1, 1978·The Journal of Comparative Neurology·M SarP Cuatrecasas
Dec 1, 1979·Journal of the Autonomic Nervous System·J D Coil, R Norgren
Jan 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A Latt, G Stetten
Jul 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A BerodJ F Pujol
Feb 15, 1980·The Journal of Comparative Neurology·D G Amaral, W M Cowan
Nov 1, 1976·Neuroscience Letters·M B Hancock
Nov 1, 1978·Neuroscience Letters·T OnoA Simpson
Feb 1, 1946·The Anatomical Record·M ABERCROMBIE

❮ Previous
Next ❯

Citations

Jan 27, 1997·The Journal of Comparative Neurology·T L Krukoff, P Khalili
Jan 1, 1982·Histochemistry·H HaraS Tsukahara
Apr 23, 1984·Brain Research·J M Wyss, M K Donovan
Jan 1, 1989·Brain Research. Brain Research Reviews·E A Fox, T L Powley
Jan 1, 1984·Journal of Neuroscience Methods·G F AlheidL Heimer
Aug 1, 1990·Journal of Neuroscience Methods·A Alvarez-BuyllaJ R Kirn
Jul 1, 1994·Journal of Neuroscience Methods·F J RichmondD B Thomson
May 1, 1982·Journal of the Autonomic Nervous System·C J Dalsgaard, L G Elfvin
Mar 1, 1984·Journal of the Autonomic Nervous System·P G LuitenA B Steffens
Jan 1, 1986·Journal of the Autonomic Nervous System·E A Fox, T L Powley
Dec 1, 1988·Journal of the Autonomic Nervous System·W R EwartB F King

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.