A Method for Visualization of Incoming Adenovirus Chromatin Complexes in Fixed and Living Cells

PloS One
Tetsuro KomatsuHarald Wodrich

Abstract

Inside the adenovirus virion, the genome forms a chromatin-like structure with viral basic core proteins. Core protein VII is the major DNA binding protein and was shown to remain associated with viral genomes upon virus entry even after nuclear delivery. It has been suggested that protein VII plays a regulatory role in viral gene expression and is a functional component of viral chromatin complexes in host cells. As such, protein VII could be used as a maker to track adenoviral chromatin complexes in vivo. In this study, we characterize a new monoclonal antibody against protein VII that stains incoming viral chromatin complexes following nuclear import. Furthermore, we describe the development of a novel imaging system that uses Template Activating Factor-I (TAF-I/SET), a cellular chromatin protein tightly bound to protein VII upon infection. This setup allows us not only to rapidly visualize protein VII foci in fixed cells but also to monitor their movement in living cells. These powerful tools can provide novel insights into the spatio-temporal regulation of incoming adenoviral chromatin complexes.

References

May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·K NagataK Matsumoto
Oct 6, 1997·The EMBO Journal·U F GreberA Helenius
Dec 21, 2006·Nucleic Acids Research·Kohsuke KatoKyosuke Nagata
Dec 18, 2008·The Journal of General Virology·W C Russell
Mar 25, 2010·PLoS Pathogens·Harald WodrichEric J Kremer
Nov 5, 2010·Journal of Virology·Daniel PuntenerUrs F Greber
Nov 26, 2011·Nucleic Acids Research·Andrea N GibersonRobin J Parks
Mar 20, 2012·PLoS Pathogens·Sabrina SchreinerHarald Wodrich
Oct 8, 2013·Nature Structural & Molecular Biology·Yusuke MiyanariMaria-Elena Torres-Padilla
Oct 22, 2013·Cell Host & Microbe·I-Hsuan WangUrs F Greber

❮ Previous
Next ❯

Citations

Mar 26, 2016·Biochemical and Biophysical Research Communications·Tetsuro KomatsuHarald Wodrich
Aug 11, 2017·Journal of Virology·Daphne C AvgoustiMatthew D Weitzman
Nov 27, 2019·FEBS Letters·Kelsey L LynchDaphne C Avgousti
Nov 24, 2019·FEBS Letters·Noémie Pied, Harald Wodrich
Nov 12, 2019·FEBS Letters·Paloma Hidalgo, Ramón A Gonzalez
Jul 15, 2020·Nature Microbiology·Christin HerrmannMatthew D Weitzman
Mar 13, 2020·Journal of Virology·Irene Carlon-AndresHarald Wodrich
Jan 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Stephanie L SarbanesCharles M Rice

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY339865.1

Methods Mentioned

BETA
immunoprecipitation
ChIP
transfection
PCR
FCS
confocal microscopy

Software Mentioned

ImageJ
MetaMorph
Addgene

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.