PMID: 38853Aug 22, 1979

A method of estimating the amount of calcium bound to the metallochromic indicator arsenazo III

Biochimica Et Biophysica Acta
S T Ohnishi


As a metallochromic indicator for ionized calcium, arsenazo III is approximately 50 times more sensitive than murexide. However, because of the high binding constant for calcium, the following problems may occur: (a) a considerable amount of calcium is bound to arsenazo III, thereby causing an error in estimating the concentration of ionized calcium; (b) the amount of bound calcium varies with the concentrations of calcium;, arsenazo III, magnesium ion and monovalent cations; (c) the amount also varies with pH, (d) the relationship between the absorbance change and the concentration of ionized calcium is nonlinear; and (e) the binding constant of arsenazo III for calcium cannot be determined by the conventional double reciprocal plot. A new experimental and theoretical method is presented which copes with these problems.


Jan 1, 1982·Progress in Biophysics and Molecular Biology·J R BlinksF G Prendergast
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J Thiele, J E Schultz
Sep 1, 1984·Archives of Biochemistry and Biophysics·S T OhnishiE Rubin
Jul 15, 1982·Analytical Biochemistry·P L DorogiE Neumann
Jul 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T KometaniS T Ohnishi
Apr 1, 1985·Cell Calcium·J Vergara, M Delay
Apr 1, 1992·Cell Calcium·S UribeJ P Pardo

Related Concepts

Calcium [EPC]
Study, Methodological
Ionized calcium
Calcium ion
Arsenazo III
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Ionized Calcium Assay
Cations, Monovalent

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