A method of screening highly common neoantigens with immunogenicity in colorectal cancer based on public somatic mutation library

Yi chuan = Hereditas
Li Li QinBo Li

Abstract

Colorectal cancer (CRC) is a malignant cancer with high incidence and mortality in the world. Immunotherapy targeting neoantigens can induce durable tumor regression in cancer patients, but is almost limited to personalized precision therapy, due to the individual differences of unique neoantigens. With the discovery of many common oncogenic mutations, and such mutation-associated neoantigens could cover more patients, and hence are valuable in clinical field. However, whether the common neoantigens can be identified in CRC is unknown. Combining the somatic mutations data from 321 CRC patients with a filter standard and 7 predicted algorithms, we screened and obtained 25 HLA-A*1101-restricted common neoantigens with a high binding affinity (IC50<50 nmol/L) and presentation score (>0.90). Besides the positive epitope KRAS_G12V8-16, 11 out of 25 common neoantigens specifically induced in vitro pre- stimulated cytotoxic lymphocyte (CTL) to secrete interferon gamma (IFN-γ). Moreover, combining cell-sorting technology and single-cell RNA sequencing, the immune repertoire profiles of C1orf170_S418G413-421 and KRAS_G12V8-16-specific CTL were analyzed and validated. Their related T-cell receptor engineered T cell (TCR-T) cells could al...Continue Reading

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Related Papers

[Rinshō ketsueki] The Japanese journal of clinical hematology
Shuichi ShinoharaHirokazu Matsushita
Revue française d'études cliniques et biologiques
G MathéJ F Doré
Seminars in Immunology
Yong-Chen Lu, Paul F Robbins
Mammalian Genome : Official Journal of the International Mammalian Genome Society
Sharon Hutchison, Antonia L Pritchard
© 2021 Meta ULC. All rights reserved