A method to develop mock arteries suitable for cell seeding and in-vitro cell culture experiments

Journal of the Mechanical Behavior of Biomedical Materials
A ColomboC Lally

Abstract

Sylgard((R)) is a biocompatible elastomer which has been widely used in biomedical applications including in simulations of the mechanical response of soft tissues and mechanotransduction investigations. In this study the effect of fabrication parameters including base to curing agent ratio and curing time on the mechanical response of Sylgard((R)) was investigated and a novel fabrication technique for the production of mock arteries with highly uniform thickness, which is essential for mechanotransduction studies, is described. Finally a method for the surface treatment of Sylgard((R)) using sulphuric acid and fibronectin to enhance smooth muscle cell (SMC) adhesion is proposed and examined in vitro. Sylgard((R)) mock coronary arteries fabricated using the proposed technique exhibited a mechanical response close to arterial tissue with cell adhesion enhanced using the surface treatment techniques described.

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Citations

Mar 19, 2016·Journal of the Mechanical Behavior of Biomedical Materials·R HopfA E Ehret
Jun 6, 2014·Computer Methods in Biomechanics and Biomedical Engineering·Jingliang DongJiyuan Tu
Oct 28, 2016·Medical & Biological Engineering & Computing·Emmet GalvinCaitríona Lally
Sep 10, 2016·Scientific Reports·Kenneth K Y HoAllen P Liu
May 10, 2018·EuroIntervention : Journal of EuroPCR in Collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology·Michael J LipinskiRon Waksman
Nov 8, 2014·Journal of Biomechanical Engineering·Caleb A DavisMichael Moreno
Mar 20, 2014·Soft Matter·Yu-Chen ChuangLi-Jen Chen
Mar 10, 2016·The International Journal of Artificial Organs·Daniel N CoakleyTim M McGloughlin

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