PMID: 9639669Jun 26, 1998Paper

A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block

Mutation Research
P Uma DeviR Kamath

Abstract

The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different tumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma (C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. It was found that three doses of 3+2+2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation.

References

Feb 1, 1985·Mutation Research·M Fenech, A A Morley

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Citations

Jan 1, 2005·Indian Journal of Clinical Biochemistry : IJCB·K ReshmaDm Vasudevan
Dec 22, 1998·Mutation Research·K K Vijayalaxmi, R Venu
Sep 28, 2010·Environmental and Molecular Mutagenesis·Stephanie CoffingMaik Schuler
Jun 18, 2004·Annals of the New York Academy of Sciences·Loren J Field

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